Rhen M, Virtanen M, Mäkelä P H
National Public Health Institute, Helsinki, Finland.
Microb Pathog. 1989 Feb;6(2):153-8. doi: 10.1016/0882-4010(89)90018-1.
The virulence-associated plasmid pEX102 of Salmonella typhimurium line TML R66 was tagged with the transposon Tn5 and the virulence of the mutants obtained was assayed in the mouse salmonellosis model. Out of 36 independent insertion mutants tested two isolates had clearly reduced virulence in (CBA x C57B1/6)F1 mice. The corresponding Tn5 elements were positioned on the restriction endonuclease map of pEX102 and found to be some 4 kilobases apart (kb) on the 96 kb virulence plasmid.
鼠伤寒沙门氏菌TML R66株的毒力相关质粒pEX102用转座子Tn5进行标记，并在小鼠沙门氏菌病模型中检测所得突变体的毒力。在测试的36个独立插入突变体中，有两个分离株在（CBA×C57B1/6）F1小鼠中的毒力明显降低。相应的Tn5元件定位在pEX102的限制性内切酶图谱上，发现在96 kb的毒力质粒上相距约4千碱基（kb）。
