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一种用于大型质粒物理和遗传图谱构建的新方法：应用于鼠伤寒沙门氏菌90 kb质粒上毒力决定簇的定位

A new method for the physical and genetic mapping of large plasmids: application to the localisation of the virulence determinants on the 90 kb plasmid of Salmonella typhimurium.

作者信息

Michiels T, Popoff M Y, Durviaux S, Coynault C, Cornelis G

机构信息

Université Catholique de Louvain, Unité de Microbiologie, Brussels, Belgium.

出版信息

Microb Pathog. 1987 Aug;3(2):109-16. doi: 10.1016/0882-4010(87)90069-6.

DOI:10.1016/0882-4010(87)90069-6
PMID:2849015
Abstract

A new method based on transposon-promoted deletions was used to generate a set of deletions in the 90 kb virulence plasmid of Salmonella typhimurium. The analysis of 16 deletion mutants allowed: (1) construction of the restriction map of the plasmid for HindIII, BamHI and BglII; (2) localisation of the plasmid region involved in virulence; (3) identification of two functional replicons on the plasmid.

摘要

一种基于转座子促进缺失的新方法被用于在鼠伤寒沙门氏菌90 kb毒力质粒中产生一系列缺失。对16个缺失突变体的分析实现了：(1)构建该质粒针对HindIII、BamHI和BglII的限制性图谱；(2)确定质粒中参与毒力的区域；(3)鉴定该质粒上的两个功能性复制子。

相似文献

1
A new method for the physical and genetic mapping of large plasmids: application to the localisation of the virulence determinants on the 90 kb plasmid of Salmonella typhimurium.一种用于大型质粒物理和遗传图谱构建的新方法：应用于鼠伤寒沙门氏菌90 kb质粒上毒力决定簇的定位
Microb Pathog. 1987 Aug;3(2):109-16. doi: 10.1016/0882-4010(87)90069-6.
2
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引用本文的文献

1
Virulence plasmid-borne spvB and spvC genes can replace the 90-kilobase plasmid in conferring virulence to Salmonella enterica serovar Typhimurium in subcutaneously inoculated mice.毒力质粒携带的spvB和spvC基因可替代90千碱基对的质粒，在皮下接种的小鼠中赋予鼠伤寒沙门氏菌毒力。
J Bacteriol. 2001 Aug;183(15):4652-8. doi: 10.1128/JB.183.15.4652-4658.2001.
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Distribution, gene sequence and expression in vivo of the plasmid encoded fimbrial antigen of Salmonella serotype Enteritidis.肠炎沙门氏菌血清型质粒编码菌毛抗原的体内分布、基因序列及表达
Epidemiol Infect. 1996 Aug;117(1):17-28. doi: 10.1017/s0950268800001084.
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The XbaI-BlnI-CeuI genomic cleavage map of Salmonella typhimurium LT2 determined by double digestion, end labelling, and pulsed-field gel electrophoresis.
通过双酶切、末端标记和脉冲场凝胶电泳确定的鼠伤寒沙门氏菌LT2的XbaI - BlnI - CeuI基因组切割图谱。
J Bacteriol. 1993 Jul;175(13):4104-20. doi: 10.1128/jb.175.13.4104-4120.1993.
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Linkage map of Salmonella typhimurium, edition VII.鼠伤寒沙门氏菌连锁图谱，第七版。
Microbiol Rev. 1988 Dec;52(4):485-532. doi: 10.1128/mr.52.4.485-532.1988.
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Hybridization studies with a DNA probe derived from the virulence region of the 60 Mdal plasmid of Salmonella typhimurium.用源自鼠伤寒沙门氏菌60 Mdal质粒毒力区的DNA探针进行杂交研究。
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