Lu Yidan, Zhang Yali, Zhou Hao, Yu Fang, Sun Shumei, Rui Yongyu
Department of Pediatrics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Nov;34(11):1697-701.
To study the in vitro antibacterial activity of meropenem combined with doxycycline, ciprofloxacin, sulbactam or cefoperazone/sulbactam against clinically isolated extensively drug-resistant Acinetobacter baumannii (XDRAB).
Using a checker board synergy design, the minimal inhibitory concentration (MIC) of antibiotics against 50 isolates of XDRAB was determined by broth microdilution antifungal susceptibility test. The fractional inhibitory concentration (FIC) index was calculated to determine the combined effect of the antibiotics.
Meropenem showed significantly reduced MIC50 and enhanced antimicrobial activities when combined with doxycycline, sulbactam or cefoperazone/sulbactam. The FIC results suggested that the main actions of doxycycline, sulbactam, and cefoperazone/sulbactam were synergistic (38%, 26%, and 10%, respectively) and addictive (62%, 74%, and 90%, respectively) without indifferent or antagonistic effects. The main actions of meropenem combined with ciprofloxacin were additive (56%) and indifference (44%) with synergistic and antagonistic effects.
Meropenem combined with doxycycline, sulbactam or cefoperazone/sulbactam shows excellent activity against clinical isolates of XDRAB.
研究美罗培南联合多西环素、环丙沙星、舒巴坦或头孢哌酮/舒巴坦对临床分离的广泛耐药鲍曼不动杆菌（XDRAB）的体外抗菌活性。
采用棋盘法协同设计，通过肉汤微量稀释法抗真菌药敏试验测定抗生素对50株XDRAB的最低抑菌浓度（MIC）。计算部分抑菌浓度（FIC）指数以确定抗生素的联合效应。
美罗培南与多西环素、舒巴坦或头孢哌酮/舒巴坦联合使用时，MIC50显著降低，抗菌活性增强。FIC结果表明，多西环素、舒巴坦和头孢哌酮/舒巴坦的主要作用为协同（分别为38%、26%和10%）和相加（分别为62%、74%和90%），无无关或拮抗作用。美罗培南与环丙沙星联合使用的主要作用为相加（56%）和无关（44%），有协同和拮抗作用。
美罗培南联合多西环素、舒巴坦或头孢哌酮/舒巴坦对临床分离的XDRAB显示出优异的活性。
