DNA条形码技术无法区分南美洲锥蝽（半翅目：猎蝽科），即恰加斯病的病媒。
DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors.
作者信息
Justi Silvia Andrade, Dale Carolina, Galvão Cleber
机构信息
Universidade Federal do Rio de Janeiro, Instituto de Biologia, Laboratório de Biologia Evolutiva Teórica e Aplicada, Rio de Janeiro, Brazil.
Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, InstitutoOswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.
出版信息
Parasit Vectors. 2014 Nov 21;7:519. doi: 10.1186/s13071-014-0519-1.
BACKGROUND
DNA barcoding assumes that a biological entity is completely separated from its closest relatives by a barcoding gap, which means that intraspecific genetic distance (from COI sequences) should never be greater than interspecific distances. We investigated the applicability of this strategy in identifying species of the genus Triatoma from South America.
FINDINGS
We calculated intra and interspecific Kimura-2-parameter distances between species from the infestans, matogrossensis, sordida and rubrovaria subcomplexes. In every subcomplex examined we observed at least one intraspecific distance greater than interspecific distances.
CONCLUSIONS
Although DNA barcoding is a straightforward approach, it was not applicable for identifying Southern American Triatoma species, which may have diverged recently. Thus, caution should be taken in identifying vector species using this approach, especially in groups where accurate identification of taxa is fundamentally linked to public health issues.
背景
DNA条形码技术假定一个生物实体通过条形码间隙与其最亲近的亲属完全分离，这意味着种内遗传距离（来自细胞色素氧化酶亚基I序列）绝不应该大于种间距离。我们研究了这种策略在识别来自南美洲的锥蝽属物种中的适用性。
研究结果
我们计算了来自感染亚复合体、马托格罗索亚复合体、污色亚复合体和红变亚复合体的物种之间的种内和种间Kimura-2-参数距离。在每个被检查的亚复合体中，我们都观察到至少一个种内距离大于种间距离。
结论
尽管DNA条形码技术是一种直接的方法，但它不适用于识别可能最近才分化的南美洲锥蝽物种。因此，在使用这种方法识别病媒物种时应谨慎，特别是在准确识别分类群与公共卫生问题密切相关的群体中。
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