Department of Biotechnology, Pukyong National University, Namku, Busan 608-737, Republic of Korea , Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Am J Chin Med. 2014;42(6):1371-84. doi: 10.1142/S0192415X14500864.
Undaria pinnatifida (Harvey) Suringar and Saccharina japonica Areschoug are two common seaweeds, and both are known to have numerous pharmacological properties that include neuroprotective effects. In a previous study, we found that the ethanol extracts of U. pinnatifida (UPE) and S. japonica (SJE) had neurite promoting activities on developing hippocampal neurons. In the present study, we studied and compared the effects of UPE and SJE on neuronal maturation. Both UPE and SJE promoted neurite outgrowth in a dose-dependent manner with optimal concentrations of 5 and 15 μg/mL, respectively. Initial neuronal differentiation was significantly promoted by UPE and SJE. Subsequently, treatment with both increased indices of axonal and dendritic cytoarchitecture, such as, the numbers and lengths of primary processes, although only UPE had a significant effect on branching frequencies. In addition, UPE and SJE showed no evidence of cytotoxicity, rather they protected neurons from naturally occurring death in vitro. These results indicate that UPE and SJE promote axodendritic maturation and neuronal survival and suggest that these algal extracts, especially UPE, have beneficial effects on the nervous system.
裙带菜（Harvey）Suringar 和昆布（Areschoug）是两种常见的海藻，都具有许多药理学特性，包括神经保护作用。在之前的研究中，我们发现裙带菜的乙醇提取物（UPE）和昆布的乙醇提取物（SJE）对发育中的海马神经元具有促进神经突生长的活性。在本研究中，我们研究并比较了 UPE 和 SJE 对神经元成熟的影响。UPE 和 SJE 均以剂量依赖性方式促进神经突生长，最佳浓度分别为 5 和 15μg/mL。UPE 和 SJE 均能显著促进初始神经元分化。随后，两种提取物均增加了轴突和树突细胞结构的指标，如初级突起的数量和长度，但只有 UPE 对分支频率有显著影响。此外，UPE 和 SJE 没有显示出细胞毒性的证据，而是在体外保护神经元免受自然死亡。这些结果表明，UPE 和 SJE 促进轴突树突成熟和神经元存活，并表明这些藻类提取物，特别是 UPE，对神经系统具有有益作用。
