Mohibbullah Md, Hannan Md Abdul, Choi Ji-Young, Bhuiyan Mohammad Maqueshudul Haque, Hong Yong-Ki, Choi Jae-Suk, Choi In Soon, Moon Il Soo
1 Department of Biotechnology, Pukyong National University , Busan, Korea.
2 Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University , Mymensingh, Bangladesh .
J Med Food. 2015 Sep;18(9):960-71. doi: 10.1089/jmf.2014.3369. Epub 2015 Jun 24.
Age-related neurological disorders are of growing concern among the elderly, and natural products with neuroprotective properties have been attracting increasing attention as candidates for the prevention or treatment of neurological disorders induced by oxidative stress. In an effort to explore natural resources, we collected some common marine seaweed from the Korean peninsula and Indonesia and screened them for neuroprotective activity against hypoxia/reoxygenation (H/R)-induced oxidative stress. Of the 23 seaweeds examined, the ethanol extract of Gracilariopsis chorda (GCE) provided maximum neuroprotection at an optimum concentration of 15 μg/mL, followed by Undaria pinnatifida. GCE increased cell viability after H/R, decreased the formation of reactive oxygen species (measured by 2',7'-dichlorodihydrofluorescein diacetate [DCF-DA] staining), and inhibited the double-stranded DNA breaks (measured by H2AX immunocytochemistry), apoptosis (measured by Annexin V/propidium iodide staining), internucleosomal DNA fragmentation (measured by DNA laddering), and dissipation of mitochondrial membrane potential (measured by JC-1 staining). Using reverse-phase high-pressure liquid chromatography, we quantitated the arachidonic acid (AA) in GCE, which provides neuroprotection against H/R-induced oxidative stress. This neuroprotective effect of AA was comparable to that of GCE. These findings suggest that the neuroprotective effect of GCE against H/R-induced neuronal death is due, at least in part, to the AA content that suppresses neuronal apoptosis.
与年龄相关的神经疾病在老年人中越来越受到关注，具有神经保护特性的天然产物作为预防或治疗由氧化应激引起的神经疾病的候选物，正吸引着越来越多的关注。为了探索自然资源，我们从朝鲜半岛和印度尼西亚收集了一些常见的海洋海藻，并筛选它们对缺氧/复氧（H/R）诱导的氧化应激的神经保护活性。在所检测的23种海藻中，细基江蓠（Gracilariopsis chorda）的乙醇提取物（GCE）在最佳浓度15μg/mL时提供了最大的神经保护作用，其次是裙带菜。GCE增加了H/R后的细胞活力，减少了活性氧的形成（通过2',7'-二氯二氢荧光素二乙酸酯[DCF-DA]染色测量），并抑制了双链DNA断裂（通过H2AX免疫细胞化学测量）、细胞凋亡（通过膜联蛋白V/碘化丙啶染色测量）、核小体间DNA片段化（通过DNA梯状条带测量）以及线粒体膜电位的耗散（通过JC-1染色测量）。使用反相高压液相色谱法，我们对GCE中的花生四烯酸（AA）进行了定量，其对H/R诱导的氧化应激具有神经保护作用。AA的这种神经保护作用与GCE相当。这些发现表明，GCE对H/R诱导的神经元死亡的神经保护作用至少部分归因于抑制神经元凋亡的AA含量。
