吐温80对出芽短梗霉CCTCC M2012223产聚苹果酸和普鲁兰多糖的影响
The effect of Tween 80 on the polymalic acid and pullulan production by Aureobasidium pullulans CCTCC M2012223.
作者信息
Tu Guangwei, Wang Yongkang, Ji Yunchao, Zou Xiang
机构信息
College of Pharmaceutical Sciences, Southwest University, 2 Tian Sheng Road, Beibei, Chongqing, 400715, People's Republic of China.
出版信息
World J Microbiol Biotechnol. 2015 Jan;31(1):219-26. doi: 10.1007/s11274-014-1779-9. Epub 2014 Nov 21.
The effect of Tween 80 on the fermentative production of polymalic acid (PMA) and pullulan using Aureobasidium pullulans CCTCC M2012223 was investigated. Tween 80 is beneficial for the biosynthesis of PMA and pullulan, and can regulate the ratio of PMA to pullulan in a dose-dependent manner. After adding 0.05 % Tween 80 to the media, the maximal PMA and pullulan production was 46.45 and 28.8 g/L at 60 h in a 5 L fermenter, with an increase of 75.08 and 27.21 % when compared to the control. Tween 80 could regulate and enhance oxygen uptake rate and carbon dioxide evolution rate in the early phase of fermentation, and change the cell morphology. The transcription levels of mitochondrial dicarboxylate transporter and transmembrane transporter were also dramatically upregulated. The present work will be helpful in deeply understanding the mechanism of Tween 80 on the effect of PMA and pullulan production.
研究了吐温80对出芽短梗霉CCTCC M2012223发酵生产聚苹果酸（PMA）和普鲁兰多糖的影响。吐温80有利于PMA和普鲁兰多糖的生物合成，并能以剂量依赖的方式调节PMA与普鲁兰多糖的比例。在培养基中添加0.05%吐温80后，在5 L发酵罐中60 h时，PMA和普鲁兰多糖的最大产量分别为46.45 g/L和28.8 g/L，与对照相比分别提高了75.08%和27.21%。吐温80可在发酵前期调节并提高氧气摄取率和二氧化碳释放率，并改变细胞形态。线粒体二羧酸转运蛋白和跨膜转运蛋白的转录水平也显著上调。本研究将有助于深入理解吐温80对PMA和普鲁兰多糖生产影响的机制。
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