Zelenin Sergey, Hansson Jonas, Ardabili Sahar, Ramachandraiah Harisha, Brismar Hjalmar, Russom Aman
Science for Life Laboratory, Division of Proteomics and Nanobiotechnology, KTH Royal Institute of Technology, Solna, PO Box 1031, 17121, Stockholm, Sweden.
Biotechnol Lett. 2015 Apr;37(4):825-30. doi: 10.1007/s10529-014-1734-8. Epub 2014 Nov 21.
Blood-stream infections (BSI) remain a major health challenge, with an increasing incidence worldwide and a high mortality rate. Early treatment with appropriate antibiotics can reduce BSI-related morbidity and mortality, but success requires rapid identification of the infecting organisms. The rapid, culture-independent diagnosis of BSI could be significantly facilitated by straightforward isolation of highly purified bacteria from whole blood. We present a microfluidic-based, sample-preparation system that rapidly and selectively lyses all blood cells while it extracts intact bacteria for downstream analysis. Whole blood is exposed to a mild detergent, which lyses most blood cells, and then to osmotic shock using deionized water, which eliminates the remaining white blood cells. The recovered bacteria are 100% viable, which opens up possibilities for performing drug susceptibility tests and for nucleic-acid-based molecular identification.
血流感染（BSI）仍然是一项重大的健康挑战，在全球范围内其发病率不断上升且死亡率很高。早期使用适当的抗生素进行治疗可以降低与BSI相关的发病率和死亡率，但成功治疗需要快速识别感染病原体。通过从全血中直接分离出高度纯化的细菌，可以显著促进对BSI进行快速、无需培养的诊断。我们展示了一种基于微流控的样品制备系统，该系统能快速且有选择性地裂解所有血细胞，同时提取完整的细菌用于下游分析。全血先接触一种温和的去污剂，该去污剂会裂解大多数血细胞，然后再用去离子水进行渗透压休克处理，以清除剩余的白细胞。回收的细菌具有100%的存活率，这为进行药敏试验和基于核酸的分子鉴定开辟了可能性。
