Wu Wenshuai, Mu Ying
Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China.
Biomicrofluidics. 2024 Jun 7;18(3):031504. doi: 10.1063/5.0190112. eCollection 2024 May.
Antimicrobial resistance is getting serious and becoming a threat to public health worldwide. The improper and excessive use of antibiotics is responsible for this situation. The standard methods used in clinical laboratories, to diagnose bacterial infections, identify pathogens, and determine susceptibility profiles, are time-consuming and labor-intensive, leaving the empirical antimicrobial therapy as the only option for the first treatment. To prevent the situation from getting worse, evidence-based therapy should be given. The choosing of effective drugs requires powerful diagnostic tools to provide comprehensive information on infections. Recent progress in microfluidics is pushing infection diagnosis and antimicrobial susceptibility testing (AST) to be faster and easier. This review summarizes the recent development in microfluidic assays for rapid identification and AST in bacterial infections. Finally, we discuss the perspective of microfluidic-AST to develop the next-generation infection diagnosis technologies.
抗菌耐药性问题日益严重，正成为全球公共卫生的一大威胁。抗生素的不当和过度使用导致了这种情况。临床实验室用于诊断细菌感染、鉴定病原体和确定药敏谱的标准方法既耗时又费力，使得经验性抗菌治疗成为首次治疗的唯一选择。为防止情况恶化，应采用循证治疗。选择有效的药物需要强大的诊断工具来提供有关感染的全面信息。微流控技术的最新进展正在推动感染诊断和抗菌药敏试验（AST）变得更快、更简便。本文综述了用于细菌感染快速鉴定和AST的微流控分析方法的最新进展。最后，我们讨论了微流控AST在开发下一代感染诊断技术方面的前景。
