针对视黄酸人类核受体α和β的特异性抗体。

Antibodies specific to the retinoic acid human nuclear receptors alpha and beta.

作者信息

Gaub M P, Lutz Y, Ruberte E, Petkovich M, Brand N, Chambon P

机构信息

Unité 184 de Biologie Moléculaire et de Génie Génétique de Institut National de la Santé et de la Recherche Médicale, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.

出版信息

Proc Natl Acad Sci U S A. 1989 May;86(9):3089-93. doi: 10.1073/pnas.86.9.3089.

DOI:10.1073/pnas.86.9.3089

PMID:2541430

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC287070/

Abstract

Two cDNAs encoding two human receptors for retinoic acid (RA), RAR-alpha and RAR-beta, have been characterized recently. Synthetic peptides corresponding to the cDNA-deduced amino acid sequences unique to RAR-alpha and RAR-beta were used to generate anti-RAR-alpha antiserum (SP171) and anti-RAR-beta antisera (SP172 and SP248). The specificity of these antisera was confirmed both by immunocytochemical detection of these receptors in COS-1 cells transfected with RAR-alpha and RAR-beta expression vectors and by immunoblot analyses performed with whole extracts of these cells. We also demonstrate that these antisera recognize RAR-alpha and RAR-beta endogenously expressed in the RA-responsive human promyelocytic leukemia cell line HL-60.

摘要

最近已鉴定出两种编码人类视黄酸（RA）受体RAR-α和RAR-β的cDNA。与RAR-α和RAR-β特有的cDNA推导氨基酸序列相对应的合成肽被用于制备抗RAR-α抗血清（SP171）和抗RAR-β抗血清（SP172和SP248）。通过用RAR-α和RAR-β表达载体转染的COS-1细胞中这些受体的免疫细胞化学检测以及用这些细胞的全提取物进行的免疫印迹分析，证实了这些抗血清的特异性。我们还证明，这些抗血清能够识别在RA反应性人类早幼粒细胞白血病细胞系HL-60中内源性表达的RAR-α和RAR-β。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d52e/287070/140d053db556/pnas00249-0109-a.jpg

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针对视黄酸人类核受体α和β的特异性抗体。

Antibodies specific to the retinoic acid human nuclear receptors alpha and beta.

作者信息

Gaub M P, Lutz Y, Ruberte E, Petkovich M, Brand N, Chambon P

机构信息

Unité 184 de Biologie Moléculaire et de Génie Génétique de Institut National de la Santé et de la Recherche Médicale, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.

出版信息

Proc Natl Acad Sci U S A. 1989 May;86(9):3089-93. doi: 10.1073/pnas.86.9.3089.

DOI:10.1073/pnas.86.9.3089

PMID:2541430

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC287070/

Abstract

摘要

针对视黄酸人类核受体α和β的特异性抗体。

Antibodies specific to the retinoic acid human nuclear receptors alpha and beta.

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机构信息

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针对视黄酸人类核受体α和β的特异性抗体。

Antibodies specific to the retinoic acid human nuclear receptors alpha and beta.

作者信息

机构信息

出版信息

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