使用单克隆抗体鉴定人、小鼠和大鼠的视黄酸受体α

Identification of human, mouse, and rat retinoic acid receptor alpha using monoclonal antibodies.

作者信息

Ali M, Torian B E, Vedeckis W V

机构信息

Gene Expression Laboratory, Pennington Biomedical Research Center, Baton Rouge, Louisiana 70808.

出版信息

Biochem Biophys Res Commun. 1992 Feb 14;182(3):1032-9. doi: 10.1016/0006-291x(92)91835-e.

DOI:10.1016/0006-291x(92)91835-e

PMID:1311565

Abstract

Monoclonal antibodies that recognize the human, mouse, and rat retinoic acid receptor alpha (RAR alpha) protein have been generated using synthetic peptides. Less well-characterized monoclonal antibodies were also generated against the RAR beta and RAR gamma proteins. Monoclonal antibodies of the IgG1 (R alpha 10) and IgG2a (R alpha 13) isotypes effectively and specifically recognize both the human and mouse RAR alpha protein. Preincubation of the antibodies with the synthetic RAR alpha peptide, but not with the RAR beta or RAR gamma peptides, blocked recognition of the approximately 55 kDa RAR alpha protein on western blots. These monoclonal antibodies also detected differing levels of RAR alpha in various rat tissues. These monoclonal antibodies will serve as powerful reagents to study the structure and regulation of the retinoic acid receptor protein.

摘要

利用合成肽已制备出可识别人类、小鼠和大鼠视黄酸受体α（RARα）蛋白的单克隆抗体。同时也制备了针对RARβ和RARγ蛋白的、特征描述较少的单克隆抗体。IgG1（Rα10）和IgG2a（Rα13）同种型的单克隆抗体可有效且特异性地识别人类和小鼠的RARα蛋白。抗体与合成的RARα肽而非RARβ或RARγ肽预孵育，可阻断蛋白质印迹法中对约55 kDa的RARα蛋白的识别。这些单克隆抗体还检测到不同大鼠组织中RARα的水平存在差异。这些单克隆抗体将作为研究视黄酸受体蛋白结构和调控的有力试剂。