Epple S, Mittmann M, Reichrath J
Department of Dermatology, University of the Saarland, Homburg, Germany.
Histochem J. 1996 Sep;28(9):657-60. doi: 10.1007/BF02331387.
A technique for the immunohistochemical detection of retinoic acid receptor alpha in cryostat sections of normal human skin in situ has been developed. A highly specific mouse monoclonal antibody, directed against the F region of retinoic acid receptor alpha, was used and a panel of 10 fixation protocols investigated. A three-step protocol, consisting of sequential fixation in 3.7% paraformaldehyde, methanol and acetone, revealed strong nuclear immunoreactivity in epidermal keratinocytes and other cell types present in normal human skin. Other fixation protocols, including fixation regimens using formaldehyde or Carnoy's solution, were less suitable or unsuitable for detection of the receptor in cryostat sections of human skin.
已开发出一种用于原位检测正常人皮肤冷冻切片中视黄酸受体α的免疫组织化学技术。使用了一种针对视黄酸受体α的F区域的高度特异性小鼠单克隆抗体，并研究了一组10种固定方案。由依次在3.7%多聚甲醛、甲醇和丙酮中固定组成的三步方案，在正常人皮肤中的表皮角质形成细胞和其他细胞类型中显示出强烈的核免疫反应性。其他固定方案，包括使用甲醛或卡诺氏液的固定方案，不太适合或不适合在人皮肤冷冻切片中检测该受体。
