• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脑脊液细胞外囊泡会经历与年龄相关的衰退，并且含有已知的和新发现的非编码RNA。

Cerebrospinal fluid extracellular vesicles undergo age dependent declines and contain known and novel non-coding RNAs.

作者信息

Tietje Ashlee, Maron Kourtney N, Wei Yanzhang, Feliciano David M

机构信息

Department of Biological Sciences, Clemson University, Clemson, South Carolina, United States of America.

出版信息

PLoS One. 2014 Nov 24;9(11):e113116. doi: 10.1371/journal.pone.0113116. eCollection 2014.

DOI:10.1371/journal.pone.0113116
PMID:25420022
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4242609/
Abstract

Brain development requires precise orchestration of cellular events through the coordinate exchange of information between distally located cells. One mechanism by which intercellular communication is achieved is through the transfer of extracellular vesicles (EVs). Exosomes are EVs that carry lipids, nucleic acids, and proteins and are detectable in most biological fluids including cerebrospinal fluid (CSF). Here we report that CSF EV concentrations undergo age dependent fluctuations. We characterized EV RNA content by next generation small RNA sequencing and miRNA microarray analysis and identified a temporal shift in CSF EV content. CSF EVs encapsulated miRNAs that contain a conserved hnRNPA2/B1 recognition sequence. We found that hnRNPA2/B1-containing EVs were produced by choroid plexus epithelial cells and that hnRNPA2/B1 containing EVs decreased with age. These results provide insight into EV exchange of miRNAs within the central nervous system and a framework to understand how changes in EVs may have an important impact on brain development.

摘要

大脑发育需要通过远距离细胞间信息的协调交换来精确编排细胞事件。实现细胞间通讯的一种机制是通过细胞外囊泡（EVs）的传递。外泌体是携带脂质、核酸和蛋白质的EVs，在包括脑脊液（CSF）在内的大多数生物体液中都可检测到。在此我们报告脑脊液EV浓度会随年龄产生波动。我们通过下一代小RNA测序和miRNA微阵列分析对EV RNA含量进行了表征，并确定了脑脊液EV含量的时间变化。脑脊液EVs包裹着含有保守hnRNPA2/B1识别序列的miRNAs。我们发现含hnRNPA2/B1的EVs由脉络丛上皮细胞产生，且含hnRNPA2/B1的EVs会随着年龄增长而减少。这些结果为深入了解中枢神经系统内miRNAs的EV交换提供了思路，并为理解EVs的变化如何可能对大脑发育产生重要影响提供了一个框架。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cdf/4242609/72734919591f/pone.0113116.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cdf/4242609/afacf864106d/pone.0113116.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cdf/4242609/ce08d5ecd743/pone.0113116.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cdf/4242609/10a644210714/pone.0113116.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cdf/4242609/609b75c66af7/pone.0113116.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cdf/4242609/72734919591f/pone.0113116.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cdf/4242609/afacf864106d/pone.0113116.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cdf/4242609/ce08d5ecd743/pone.0113116.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cdf/4242609/10a644210714/pone.0113116.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cdf/4242609/609b75c66af7/pone.0113116.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cdf/4242609/72734919591f/pone.0113116.g005.jpg

相似文献

1
Cerebrospinal fluid extracellular vesicles undergo age dependent declines and contain known and novel non-coding RNAs.脑脊液细胞外囊泡会经历与年龄相关的衰退，并且含有已知的和新发现的非编码RNA。
PLoS One. 2014 Nov 24;9(11):e113116. doi: 10.1371/journal.pone.0113116. eCollection 2014.
2
Small RNA deep sequencing discriminates subsets of extracellular vesicles released by melanoma cells--Evidence of unique microRNA cargos.小RNA深度测序可区分黑色素瘤细胞释放的细胞外囊泡亚群——独特微小RNA载荷的证据
RNA Biol. 2015;12(8):810-23. doi: 10.1080/15476286.2015.1056975.
3
Human extracellular vesicles and correlation with two clinical forms of toxoplasmosis.人细胞外囊泡与两种临床型弓形虫病的相关性。
PLoS One. 2020 Mar 3;15(3):e0229602. doi: 10.1371/journal.pone.0229602. eCollection 2020.
4
miRNA contents of cerebrospinal fluid extracellular vesicles in glioblastoma patients.胶质母细胞瘤患者脑脊液细胞外囊泡中的微小RNA含量
J Neurooncol. 2015 Jun;123(2):205-16. doi: 10.1007/s11060-015-1784-3. Epub 2015 Apr 23.
5
Identification of a novel mechanism of blood-brain communication during peripheral inflammation via choroid plexus-derived extracellular vesicles.通过脉络丛衍生的细胞外囊泡确定外周炎症期间血脑通讯的新机制。
EMBO Mol Med. 2016 Oct 4;8(10):1162-1183. doi: 10.15252/emmm.201606271. Print 2016 Oct.
6
Extracellular vesicles and their content in bioactive lipid mediators: more than a sack of microRNA.细胞外囊泡及其生物活性脂质介质中的内容物：不仅仅是 microRNA 的一个袋子。
J Lipid Res. 2018 Nov;59(11):2037-2046. doi: 10.1194/jlr.R084640. Epub 2018 Apr 20.
7
[Characteristics of exosomes andmicroparticles discovered in human tears].[在人眼泪中发现的外泌体和微颗粒的特征]
Biomed Khim. 2016 Jan-Feb;62(1):99-106. doi: 10.18097/PBMC20166201099.
8
Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.脑脊液细胞外囊泡的蛋白质组学分析：一个综合数据集。
J Proteomics. 2014 Jun 25;106:191-204. doi: 10.1016/j.jprot.2014.04.028. Epub 2014 Apr 24.
9
Extracellular vesicles: their role in cancer biology and epithelial-mesenchymal transition.细胞外囊泡：它们在癌症生物学和上皮-间质转化中的作用
Biochem J. 2017 Jan 1;474(1):21-45. doi: 10.1042/BCJ20160006.
10
Human bone marrow- and adipose-mesenchymal stem cells secrete exosomes enriched in distinctive miRNA and tRNA species.人骨髓间充质干细胞和脂肪间充质干细胞分泌富含独特微小RNA（miRNA）和转运RNA（tRNA）种类的外泌体。
Stem Cell Res Ther. 2015 Jul 1;6(1):127. doi: 10.1186/s13287-015-0116-z.

引用本文的文献

1
Apocrine secretion by the choroid plexus.脉络丛的顶浆分泌。
Fluids Barriers CNS. 2025 Jul 16;22(1):75. doi: 10.1186/s12987-025-00684-3.
2
Harnessing the circadian nature of the choroid plexus and cerebrospinal fluid.利用脉络丛和脑脊液的昼夜节律特性。
NPJ Biol Timing Sleep. 2025;2(1):19. doi: 10.1038/s44323-025-00033-5. Epub 2025 May 26.
3
Unique Patterns of Circulating Extracellular Vesicles in Preterm Infants During Adaptation to Extra-Uterine Life.早产儿适应宫外生活期间循环细胞外囊泡的独特模式。

本文引用的文献

1
Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.脑脊液细胞外囊泡的蛋白质组学分析：一个综合数据集。
J Proteomics. 2014 Jun 25;106:191-204. doi: 10.1016/j.jprot.2014.04.028. Epub 2014 Apr 24.
2
Embryonic cerebrospinal fluid nanovesicles carry evolutionarily conserved molecules and promote neural stem cell amplification.胚胎脑脊液纳米囊泡携带进化上保守的分子并促进神经干细胞扩增。
PLoS One. 2014 Feb 12;9(2):e88810. doi: 10.1371/journal.pone.0088810. eCollection 2014.
3
Sumoylated hnRNPA2B1 controls the sorting of miRNAs into exosomes through binding to specific motifs.
J Extracell Vesicles. 2025 Apr;14(4):e70064. doi: 10.1002/jev2.70064.
4
Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies.关于脑脊液细胞外囊泡研究可重复性的建议。
J Extracell Vesicles. 2024 Jan;13(1):e12397. doi: 10.1002/jev2.12397.
5
Deregulation of exosomal miRNAs in rheumatoid arthritis patients.类风湿关节炎患者外泌体 miRNAs 的失调。
PLoS One. 2023 Jul 27;18(7):e0289301. doi: 10.1371/journal.pone.0289301. eCollection 2023.
6
Neuronal Prosurvival Role of Ceramide Synthase 2 by Olidogendrocyte-to-Neuron Extracellular Vesicle Transfer.神经酰胺合酶 2 通过少突胶质细胞-神经元细胞外囊泡转移的神经元存活作用。
Int J Mol Sci. 2023 Mar 22;24(6):5986. doi: 10.3390/ijms24065986.
7
The Double-Edged Role of Extracellular Vesicles in the Hallmarks of Aging.细胞外囊泡在衰老标志中的双刃剑作用。
Biomolecules. 2023 Jan 13;13(1):165. doi: 10.3390/biom13010165.
8
Extracellular vesicles derived from the choroid plexus trigger the differentiation of neural stem cells.脉络丛来源的细胞外囊泡触发神经干细胞的分化。
J Extracell Vesicles. 2022 Nov;11(11):e12276. doi: 10.1002/jev2.12276.
9
Exosomal miRNAs as a Promising Source of Biomarkers in Colorectal Cancer Progression.外泌体 miRNA 作为结直肠癌进展中具有前景的生物标志物来源。
Int J Mol Sci. 2022 Apr 27;23(9):4855. doi: 10.3390/ijms23094855.
10
Extracellular Vesicles from Human Cerebrospinal Fluid Are Effectively Separated by Sepharose CL-6B-Comparison of Four Gravity-Flow Size Exclusion Chromatography Methods.通过琼脂糖CL-6B有效分离人脑脊液中的细胞外囊泡——四种重力流尺寸排阻色谱方法的比较
Biomedicines. 2022 Mar 27;10(4):785. doi: 10.3390/biomedicines10040785.
SUMO 化 hnRNPA2B1 通过结合特定基序控制 miRNA 分选到外泌体中。
Nat Commun. 2013;4:2980. doi: 10.1038/ncomms3980.
4
MiR-21 in the extracellular vesicles (EVs) of cerebrospinal fluid (CSF): a platform for glioblastoma biomarker development.脑脊液（CSF）细胞外囊泡（EVs）中的MiR-21：胶质母细胞瘤生物标志物开发的一个平台
PLoS One. 2013 Oct 21;8(10):e78115. doi: 10.1371/journal.pone.0078115. eCollection 2013.
5
Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity.癌细胞外泌体依赖细胞表面的硫酸乙酰肝素蛋白聚糖来实现内化及其功能活性。
Proc Natl Acad Sci U S A. 2013 Oct 22;110(43):17380-5. doi: 10.1073/pnas.1304266110. Epub 2013 Oct 7.
6
LRRK2 secretion in exosomes is regulated by 14-3-3.LRRK2 在 exosomes 中的分泌受 14-3-3 的调节。
Hum Mol Genet. 2013 Dec 15;22(24):4988-5000. doi: 10.1093/hmg/ddt346. Epub 2013 Jul 25.
7
BEAMing and Droplet Digital PCR Analysis of Mutant IDH1 mRNA in Glioma Patient Serum and Cerebrospinal Fluid Extracellular Vesicles.对胶质瘤患者血清和脑脊液细胞外囊泡中突变 IDH1 mRNA 的 BEAMing 和液滴数字 PCR 分析。
Mol Ther Nucleic Acids. 2013 Jul 23;2(7):e109. doi: 10.1038/mtna.2013.28.
8
Choroid plexus transcytosis and exosome shuttling deliver folate into brain parenchyma.脉络丛细胞的转胞吞作用和外泌体转运将叶酸递送至脑实质。
Nat Commun. 2013;4:2123. doi: 10.1038/ncomms3123.
9
A comparative study of submicron particle sizing platforms: accuracy, precision and resolution analysis of polydisperse particle size distributions.亚微米颗粒粒度分析平台的比较研究：多分散颗粒粒度分布的准确性、精密度和分辨率分析。
J Colloid Interface Sci. 2013 Sep 1;405:322-30. doi: 10.1016/j.jcis.2013.02.030. Epub 2013 Mar 1.
10
Analysis Tool Web Services from the EMBL-EBI.EMBL-EBI 的分析工具 Web 服务。
Nucleic Acids Res. 2013 Jul;41(Web Server issue):W597-600. doi: 10.1093/nar/gkt376. Epub 2013 May 13.