一种神经母细胞瘤细胞系和生殖细胞肿瘤细胞系的N-myc扩增及神经元特异性烯醇化酶产生

N-myc amplification and neuron-specific enolase production of a neuroblastoma cell line and germ cell tumor cell lines.

作者信息

Ishiwata I, Ishiwata C, Soma M, Ono I, Nakaguchi T, Nozawa S, Ishikawa H

机构信息

Ishiwata Obstetrics and Gynecologic Hospital, Ibaraki-ken, Japan.

出版信息

Gynecol Oncol. 1989 Jun;33(3):356-9. doi: 10.1016/0090-8258(89)90527-1.

DOI:10.1016/0090-8258(89)90527-1

PMID:2542141

Abstract

N-myc gene amplification of the gynecological malignant tumor cell lines and a neuroblastoma cell line was studied by the Southern hybridization method along with the production of neuron-specific enolase (NSE) by these cell lines. N-myc amplification and NSE production were observed side by side in three cell lines: neuroblastoma cell line HSNB, endodermal sinus tumor cell line HAEST, and malignant teratoma cell line HUOT. However, N-myc amplification and NSE production disappeared gradually following successive passages of the HAEST and HUOT lines. With respect to the HUOT line, these parameters disappeared along with the cells of nervous origin. N-myc amplification and NSE production were not observed in nine other cell lines.

摘要

采用Southern杂交法研究了妇科恶性肿瘤细胞系和一种神经母细胞瘤细胞系的N-myc基因扩增情况，同时研究了这些细胞系中神经元特异性烯醇化酶（NSE）的产生。在三种细胞系中同时观察到N-myc扩增和NSE产生：神经母细胞瘤细胞系HSNB、内胚窦瘤细胞系HAEST和恶性畸胎瘤细胞系HUOT。然而，随着HAEST和HUOT系连续传代，N-myc扩增和NSE产生逐渐消失。对于HUOT系，这些参数随着神经源性细胞一起消失。在其他九种细胞系中未观察到N-myc扩增和NSE产生。