Tsuchida Y, Kanda N, Shimatake H, Kaneko Y, Notomi T
Department of Surgery, National Children's Hospital, Tokyo, Japan.
Exp Cell Biol. 1988;56(5):277-84. doi: 10.1159/000163491.
Tumor doubling time, sensitivity to chemotherapeutic agents and concentrations of neuron-specific enolase were studied in nine human neuroblastoma xenografts, in which amplifications of N-myc, clones 8 and G21 were known; N-myc was amplified in eight, clone 8 in five and clone G21 in four of these nine xenografts. Tumor doubling time was longest in one xenograft, TNB10, which lacks the amplification of either N-myc or clone 8 or G21, and shortest in TNB1 in which all three DNA sequences are amplified with a DNA rearrangement in clone 8. No correlations were found between genomic amplification of N-myc, clones 8 and G21 and effectiveness of five chemotherapeutic drugs tested, except for cis-platinum. cis-Platinum was found to be effective on all but the one xenograft, TNB10, with the longest tumor doubling time. Concentration of neuron-specific enolase in tumor extract was lowest in TNB1 and correlated with the length of the tumor doubling time.
在9个人类神经母细胞瘤异种移植瘤中研究了肿瘤倍增时间、对化疗药物的敏感性以及神经元特异性烯醇化酶的浓度，已知其中N - myc、克隆8和G21存在扩增；在这9个异种移植瘤中，8个有N - myc扩增，5个有克隆8扩增，4个有克隆G21扩增。肿瘤倍增时间在一个缺乏N - myc、克隆8或G21扩增的异种移植瘤TNB10中最长，而在TNB1中最短，TNB1中所有三个DNA序列均有扩增且克隆8存在DNA重排。除顺铂外，未发现N - myc、克隆8和G21的基因组扩增与所测试的五种化疗药物的疗效之间存在相关性。发现顺铂对除肿瘤倍增时间最长的异种移植瘤TNB10外的所有异种移植瘤均有效。肿瘤提取物中神经元特异性烯醇化酶的浓度在TNB1中最低，且与肿瘤倍增时间的长短相关。
