Scott J N, Jennes L H
Department of Anatomy, Wright State University, Dayton, OH 45435.
Histochemistry. 1989;91(5):395-400. doi: 10.1007/BF00493826.
With in vitro autoradiography, specific receptors for atrial natriuretic peptide (ANP) were localized in fetal rat kidney and adrenal glands. Receptors were present over renal vesicles, in the primitive renal medulla and throughout the adrenal gland as early as 16 days gestation. By 20 days gestation, several layers of developing renal corpuscles were present and ANP receptors were localized over developing glomeruli in each layer. Larger accumulations occurred over the juxtamedullary glomeruli. In the medulla, the receptors were localized in a reticular pattern near the pelvis. With emulsion coated sections, ANP receptors in developing renal corpuscles were seen primarily over the lower curve of S-shaped vesicles and around the periphery of the more mature corpuscles. In the renal medulla, receptors were localized over the interstitial cells. In the 16-day-old adrenal gland, ANP receptors were present throughout the cortical area but at 20 days gestation and 1 day postpartum receptors appeared more numerous in the peripheral region. These data suggest that ANP has important developmental effects in the kidney and adrenal gland and may be involved in regulation of body fluid homeostasis in the late gestation rat fetus.
通过体外放射自显影技术，心房利钠肽（ANP）的特异性受体定位于胎鼠肾脏和肾上腺。早在妊娠16天时，受体就存在于肾小泡、原始肾髓质以及整个肾上腺。到妊娠20天时，出现了几层发育中的肾小体，ANP受体定位于每层发育中的肾小球上。在近髓肾小球上有较大的聚集。在髓质中，受体以网状模式定位于肾盂附近。在涂有乳剂的切片上，发育中的肾小体中的ANP受体主要见于S形小泡的下曲线以及更成熟肾小体的周边。在肾髓质中，受体定位于间质细胞上。在16日龄的肾上腺中，ANP受体存在于整个皮质区域，但在妊娠20天时和产后1天，受体在周边区域似乎更多。这些数据表明，ANP在肾脏和肾上腺的发育中具有重要作用，可能参与妊娠晚期胎鼠的体液稳态调节。
