透明质酸、HAS1 - 2和HYAL1 - 2在口腔扁平苔藓中的表达改变。

Altered expression of hyaluronan, HAS1-2, and HYAL1-2 in oral lichen planus.

作者信息

Siponen Maria, Kullaa Arja, Nieminen Pentti, Salo Tuula, Pasonen-Seppänen Sanna

机构信息

Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, Oulu, Finland.

Department of Oral and Maxillofacial Diseases, Kuopio University Hospital, Kuopio, Finland.

出版信息

J Oral Pathol Med. 2015 Jul;44(6):401-9. doi: 10.1111/jop.12294. Epub 2014 Nov 25.

DOI:10.1111/jop.12294

PMID:25421996

Abstract

BACKGROUND

Oral lichen planus (OLP) is an immune-mediated mucosal disease of unclear etiology and of unresolved pathogenesis. Hyaluronan (HA) is an extracellular matrix glycosaminoglycan involved in inflammation and tumor progression. However, its presence in OLP has not been reported. We therefore aimed to study the immunohistochemical expression of HA, its receptor CD44, hyaluronan synthases (HAS1-3), and hyaluronidases (HYAL1-2) in OLP.

METHODS

The presence of HA, CD44, HAS1-3, and HYAL1-2 was studied by immunohistochemical methods in 55 OLP and 23 control oral mucosal specimens (CTR). The localization, intensity, and differences of the epithelial expression between OLP and CTRs were analyzed.

RESULTS

HA and CD44 were found on cell membranes in the epithelial basal and intermediate layers in CTR and OLP specimens. The HA staining intensity was stronger in the basal layer of the epithelium in OLP than in CTRs (P < 0.001). HAS1 (P = 0.001) and HAS2 (P < 0.001) showed stronger staining in the basal and weaker staining in the superficial (P < 0.001) epithelial layers in OLP than in CTRs. The immunostaining of HAS3 was low in both OLP and CTRs. Positive HYAL1 and HYAL2 staining were mainly found in the basal and intermediate epithelial layers, and their intensities were significantly increased in OLP, except HYAL 2 in the intermediate epithelial layer.

CONCLUSIONS

HA, HAS1-2, and HYAL1-2 have altered expression in OLP compared to CTRs and may therefore have a role in OLP pathogenesis.

摘要

背景

口腔扁平苔藓（OLP）是一种病因不明、发病机制尚未明确的免疫介导性黏膜疾病。透明质酸（HA）是一种参与炎症和肿瘤进展的细胞外基质糖胺聚糖。然而，其在OLP中的存在尚未见报道。因此，我们旨在研究HA、其受体CD44、透明质酸合成酶（HAS1 - 3）和透明质酸酶（HYAL1 - 2）在OLP中的免疫组化表达。

方法

采用免疫组化方法研究55例OLP和23例对照口腔黏膜标本（CTR）中HA、CD44、HAS1 - 3和HYAL1 - 2的存在情况。分析OLP和CTR之间上皮表达的定位、强度及差异。

结果

在CTR和OLP标本的上皮基底层和中间层细胞膜上发现了HA和CD44。OLP上皮基底层的HA染色强度高于CTR（P < 0.001）。与CTR相比，OLP中HAS1（P = 0.001）和HAS2（P < 0.001）在上皮基底层染色较强，在表层上皮（P < 0.001）染色较弱。OLP和CTR中HAS3的免疫染色均较低。HYAL1和HYAL2阳性染色主要见于上皮基底层和中间层，除中间层的HYAL 2外，它们在OLP中的强度均显著增加。