School of International Education, Zhengzhou University, Zhengzhou, Henan Province, China ; Institute of Clinical Anatomy, Southern Medical University, Guangzhou, Guangdong Province, China.
Department of Orthopedic Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
Neural Regen Res. 2014 Oct 15;9(20):1814-9. doi: 10.4103/1673-5374.143427.
In this study, we chemically extracted acellular nerve allografts from bilateral sciatic nerves, and repaired 10-mm sciatic nerve defects in rats using these grafts and brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells. Experiments were performed in three groups: the acellular nerve allograft bridging group, acellular nerve allograft + bone marrow mesenchymal stem cells group, and the acellular nerve allograft + brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells group. Results showed that at 8 weeks after bridging, sciatic functional index, triceps wet weight recovery rate, myelin thickness, and number of myelinated nerve fibers were significantly changed in the three groups. Variations were the largest in the acellular nerve allograft + brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells group compared with the other two groups. Experimental findings suggest that chemically extracted acellular nerve allograft combined nerve factor and mesenchymal stem cells can promote the restoration of sciatic nerve defects. The repair effect seen is better than the single application of acellular nerve allograft or acellular nerve allograft combined mesenchymal stem cell transplantation.
在这项研究中，我们从双侧坐骨神经中化学提取去细胞神经同种异体移植物，并使用这些移植物和脑源性神经营养因子转染的骨髓间充质干细胞修复大鼠 10mm 的坐骨神经缺损。实验在三组中进行：去细胞神经同种异体桥接组、去细胞神经同种异体+骨髓间充质干细胞组和去细胞神经同种异体+脑源性神经营养因子转染骨髓间充质干细胞组。结果显示，在桥接后 8 周，三组的坐骨功能指数、三头肌湿重恢复率、髓鞘厚度和有髓神经纤维数量均发生明显变化。与其他两组相比，去细胞神经同种异体+脑源性神经营养因子转染骨髓间充质干细胞组的变化最大。实验结果表明，化学提取的去细胞神经同种异体与神经因子和间充质干细胞联合应用可促进坐骨神经缺损的修复。所观察到的修复效果优于单纯应用去细胞神经同种异体或去细胞神经同种异体联合骨髓间充质干细胞移植。
