Huo Ying-Yi, You Hong, Li Zheng-Yang, Wang Chun-Sheng, Xu Xue-Wei
Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
Int J Syst Evol Microbiol. 2015 Feb;65(Pt 2):676-680. doi: 10.1099/ijs.0.070433-0. Epub 2014 Nov 25.
A Gram-stain-negative, aerobic, short rod-shaped bacterium, strain LA53(T), was isolated from a deep-sea water sample collected from the eastern Pacific Ocean. Strain LA53(T) grew in the presence of 0-7.0 % (w/v) NaCl and at 15-37 °C; optimum growth was observed with 1.0-2.0 % (w/v) NaCl and at 35 °C. Chemotaxonomic analysis showed ubiquinone-10 as the predominant respiratory quinone, C18 : 1ω7c and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c) as major fatty acids, and diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid as major polar lipids. The genomic DNA G+C content was 57.7 mol%. Phylogenetic analyses revealed that strain LA53(T) belongs to the genus Novosphingobium. 16S rRNA gene sequence similarities between strain LA53(T) and the type strains of species of the genus Novosphingobium with validly published names ranged from 93.1 to 96.3 %. In addition, strain LA53(T) could be differentiated from Novosphingobium pentaromativorans DSM 17173(T) and Novosphingobium indicum DSM 23608(T) as well as the type strain of the type species of the genus, Novosphingobium capsulatum DSM 30196(T), by some phenotypic characteristics, including hydrolysis of substrates, utilization of carbon sources and susceptibility to antibiotics. On the basis of phenotypic and genotypic data, strain LA53(T) represents a novel species within the genus Novosphingobium, for which the name Novosphingobium marinum sp. nov. is proposed. The type strain is LA53(T) ( = CGMCC 1.12918(T) = JCM 30307(T)).
从东太平洋采集的深海水样中分离出一株革兰氏染色阴性、需氧、短杆状细菌，菌株LA53(T)。菌株LA53(T)在0-7.0%（w/v）NaCl存在下及15-37℃生长；在1.0-2.0%（w/v）NaCl及35℃观察到最佳生长。化学分类分析表明，泛醌-10为主要呼吸醌，C18 : 1ω7c和特征性总和3（异-C15 : 0 2-OH和/或C16 : 1ω7c）为主要脂肪酸，双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和鞘糖脂为主要极性脂质。基因组DNA G+C含量为57.7 mol%。系统发育分析表明，菌株LA53(T)属于新鞘氨醇菌属。菌株LA53(T)与有效发表名称的新鞘氨醇菌属各物种模式菌株之间的16S rRNA基因序列相似性范围为93.1%至96.3%。此外，菌株LA53(T)可通过一些表型特征与多环芳烃新鞘氨醇菌DSM 17173(T)、印度新鞘氨醇菌DSM 23608(T)以及该属模式种的模式菌株荚膜新鞘氨醇菌DSM 30196(T)区分开来，这些表型特征包括底物水解、碳源利用和对抗生素的敏感性。基于表型和基因型数据，菌株LA53(T)代表新鞘氨醇菌属内的一个新物种，为此提议将其命名为海洋新鞘氨醇菌（Novosphingobium marinum sp. nov.）。模式菌株为LA53(T)（= CGMCC 1.12918(T)= JCM 30307(T)）。
