Department of Environmental Biotechnology, KRIBB School of Biotechnology, University of Science and Technology, Daejeon 34113, Republic of Korea.
Cell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Nov;70(11):5911-5917. doi: 10.1099/ijsem.0.004493. Epub 2020 Oct 9.
A novel Gram-stain-negative, aerobic, and rod-shaped bacterial strain, M24A2M, was isolated from seawater in the Republic of Korea. On the basis of the 16S rRNA gene phylogeny, strain M24A2M was found to be closely related to US6-1 and SM117 with pair-wise sequence similarities of 97.4 and 96.9 %, respectively. Phylogenetic analysis of 16S rRNA sequences indicated that M24A2M formed a branch with US6-1 and represented a member of the genus . The predominant cellular fatty acids were C 2-OH, summed feature 3 (C 7 and/or C 6), and summed feature 8 (C 7 and/or C 6). The polar lipids of strain M24A2M consisted mainly of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unidentified phospholipid, and two unidentified lipids. The respiratory quinone was ubiquinone Q-10. The genomic DNA G+C content was 63.9 %. Given the phenotypic characteristics along with the phylogenetic distinctness and chemotaxonomic features, strain M24A2M is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain of sp. nov. is M24A2M (=KCTC 72894=JCM 33983).
一株新型革兰氏阴性、需氧、杆状细菌，M24A2M，从韩国海域海水中分离得到。基于 16S rRNA 基因系统发育树，菌株 M24A2M 与 US6-1 和 SM117 最为接近，序列相似度分别为 97.4%和 96.9%。16S rRNA 序列的系统发育分析表明，M24A2M 与 US6-1 形成一个分支，代表 属的一个成员。主要的细胞脂肪酸为 C 2-OH、特征 3 总和（C 7 和/或 C 6）和特征 8 总和（C 7 和/或 C 6）。菌株 M24A2M 的极性脂主要由二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱、一种未鉴定的磷脂和两种未鉴定的脂组成。呼吸醌为泛醌 Q-10。基因组 DNA G+C 含量为 63.9%。鉴于表型特征、系统发育差异和化学分类特征，菌株 M24A2M 被认为代表 属中的一个新型物种，因此建议命名为 sp. nov.。该新种的模式菌株为 M24A2M（=KCTC 72894=JCM 33983）。
