Tashiro Yohei, Rodriguez Gabriel M, Atsumi Shota
Department of Chemistry, University of California-Davis, Davis, CA, USA.
J Ind Microbiol Biotechnol. 2015 Mar;42(3):361-73. doi: 10.1007/s10295-014-1547-8. Epub 2014 Nov 26.
Global energy and environmental concerns have driven the development of biological chemical production from renewable sources. Biological processes using microorganisms are efficient and have been traditionally utilized to convert biomass (i.e., glucose) to useful chemicals such as amino acids. To produce desired fuels and chemicals with high yield and rate, metabolic pathways have been enhanced and expanded with metabolic engineering and synthetic biology approaches. 2-Keto acids, which are key intermediates in amino acid biosynthesis, can be converted to a wide range of chemicals. 2-Keto acid pathways were engineered in previous research efforts and these studies demonstrated that 2-keto acid pathways have high potential for novel metabolic routes with high productivity. In this review, we discuss recently developed 2-keto acid-based pathways.
全球能源和环境问题推动了可再生资源生物化学生产的发展。利用微生物的生物过程效率很高，并且传统上已被用于将生物质（即葡萄糖）转化为有用的化学品，如氨基酸。为了高产率、高速度地生产所需的燃料和化学品，已通过代谢工程和合成生物学方法增强和扩展了代谢途径。2-酮酸是氨基酸生物合成中的关键中间体，可转化为多种化学品。在先前的研究中对2-酮酸途径进行了工程改造，这些研究表明2-酮酸途径在具有高生产率的新型代谢途径方面具有很高的潜力。在本综述中，我们讨论了最近开发的基于2-酮酸的途径。
