Su Haifeng, Lin JiaFu
Key Laboratory of Degraded and Unused Land Consolidation Engineering, The Ministry of Natural and Resources, Xian, 710075, Shanxi, China.
Antibiotics Research and Re-Evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu University, Chengdu, 610106, China.
Biotechnol Biofuels Bioprod. 2023 Jul 4;16(1):109. doi: 10.1186/s13068-023-02340-0.
Because the thermodynamic property is closer to gasoline, advanced biofuels (C ≥ 6) are appealing for replacing non-renewable fossil fuels using biosynthesis method that has presented a promising approach. Synthesizing advanced biofuels (C ≥ 6), in general, requires the expansion of carbon chains from three carbon atoms to more than six carbon atoms. Despite some specific biosynthesis pathways that have been developed in recent years, adequate summary is still lacking on how to obtain an effective metabolic pathway. Review of biosynthesis pathways for expanding carbon chains will be conducive to selecting, optimizing and discovering novel synthetic route to obtain new advanced biofuels. Herein, we first highlighted challenges on expanding carbon chains, followed by presentation of two biosynthesis strategies and review of three different types of biosynthesis pathways of carbon chain expansion for synthesizing advanced biofuels. Finally, we provided an outlook for the introduction of gene-editing technology in the development of new biosynthesis pathways of carbon chain expansion.
由于热力学性质更接近汽油，高级生物燃料（C≥6）对于使用生物合成方法替代不可再生化石燃料具有吸引力，该方法已呈现出一种有前景的途径。一般来说，合成高级生物燃料（C≥6）需要将碳链从三个碳原子扩展到六个以上碳原子。尽管近年来已经开发了一些特定的生物合成途径，但对于如何获得有效的代谢途径仍缺乏充分的总结。回顾碳链扩展的生物合成途径将有助于选择、优化和发现新的合成路线以获得新的高级生物燃料。在此，我们首先强调了碳链扩展面临的挑战，接着介绍了两种生物合成策略，并回顾了用于合成高级生物燃料的三种不同类型的碳链扩展生物合成途径。最后，我们对基因编辑技术在碳链扩展新生物合成途径开发中的引入进行了展望。
