Tatar Demet, Sahin Nevzat
Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, Kurupelit, 55139, Samsun, Turkey.
Antonie Van Leeuwenhoek. 2015 Feb;107(2):411-8. doi: 10.1007/s10482-014-0339-5. Epub 2014 Nov 27.
A novel actinomycete, designated strain B1041(T), isolated from soil collected from the Tuz (Salt) Lake in the central Anatolia region, in Turkey, was characterised using a polyphasic taxonomic approach. The isolate was found to have chemical and morphological properties typical of the members of the genus Streptomyces and formed a distinct phyletic line in the 16S rRNA gene tree. Strain B1041(T) was found to be most closely related to Streptomyces plumbiresistens CCNWHX 13-160(T) (98.46 % sequence similarity), Streptomyces pseudovenezuelae NBRC 12904(T) (97.81 %), Streptomyces novaecaesareae NBRC 13368(T) (97.68 %), Streptomyces graminifolii JL-22(T) (97.60 %), Streptomyces phaeoluteigriseus NRRL ISP-5182(T) (97.58 %), Streptomyces ciscaucasicus NBRC 12872(T) (97.53 %) and Streptomyces pratensis ch24(T) (97.52 %). Sequence similarities with other strains of the genus Streptomyces were lower than 97.5 %. The cell wall of the novel strain was found to contain LL-diaminopimelic acid and the whole cell sugars were identified as galactose, glucose and ribose. The major cellular fatty acids were identified as anteiso C15:0 , iso C16:0 and anteiso C17:0. The predominant menaquinones found were found to be MK-9(H8) and MK-9(H6). The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, a phosphoglycolipid and two unknown phospholipids. The genomic DNA G+C content was determined to be 70.4 mol%. On the basis of the data from this polyphasic taxonomic study, strain B1041(T) can be considered to represent a novel species within the genus Streptomyces for which the name Streptomyces seymenliensis sp. nov. is proposed (type strain B1041(T) =KCTC 29245(T) = DSM 42117(T)).
从土耳其安纳托利亚中部地区图兹（盐）湖采集的土壤中分离出一株新型放线菌，命名为菌株B1041(T)，采用多相分类方法对其进行了表征。该分离株具有链霉菌属成员典型的化学和形态学特性，在16S rRNA基因树中形成了一个独特的系统发育分支。发现菌株B1041(T)与铅抗性链霉菌CCNWHX 13-160(T)（序列相似性98.46%）、假委内瑞拉链霉菌NBRC 12904(T)（97.81%）、新凯撒链霉菌NBRC 13368(T)（97.68%）、禾本科链霉菌JL-22(T)（97.60%）、淡紫灰链霉菌NRRL ISP-5182(T)（97.58%）、西高加索链霉菌NBRC 12872(T)（97.53%）和草地链霉菌ch24(T)（97.52%）关系最为密切。与链霉菌属其他菌株的序列相似性低于97.5%。发现该新型菌株的细胞壁含有LL-二氨基庚二酸，全细胞糖被鉴定为半乳糖、葡萄糖和核糖。主要的细胞脂肪酸被鉴定为anteiso C15:0、iso C16:0和anteiso C17:0。发现主要的甲基萘醌为MK-9(H8)和MK-9(H6)。检测到的极性脂质为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、一种磷酸糖脂和两种未知的磷脂。基因组DNA G+C含量测定为70.4 mol%。基于这项多相分类研究的数据，菌株B1041(T)可被认为代表链霉菌属内的一个新物种，为此提出名为西梅恩链霉菌（Streptomyces seymenliensis）的新种（模式菌株B1041(T)=KCTC 29245(T)=DSM 42117(T)）。
