Microbial Biotechnology Research Laboratory, Department of Biochemistry, Manipur University, Canchipur, Imphal, 795003, Manipur, India,
Antonie Van Leeuwenhoek. 2013 Dec;104(6):1135-41. doi: 10.1007/s10482-013-0035-x. Epub 2013 Sep 14.
A strain of Streptomyces, MBRL 179(T), isolated from a sample from a Limestone quarry located at Hundung, Manipur, India, was characterized by polyphasic taxonomy. The strain formed a monophyletic clade with Streptomyces spinoverrucosus NBRC 14228(T) (16S rRNA gene sequence similarity of 99.3 %) in the Neighbour-joining tree. DNA-DNA hybridization experiment gave a DNA-DNA relatedness value of 34.7 % between MBRL 179(T) and S. spinoverrucosus NBRC 14228(T). Strain MBRL 179(T) contained LL-diaminopimelic acid, xylose, glucose, and mannose in the whole cell-wall hydrolysates along with small amount of ribose. The major polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside, with other unknown phospholipids and aminophospholipid. MK-9(H6), MK-9(H8) and MK-9(H4) were the predominant menaquinones detected. The major fatty acids were anteiso-C16:0 (28.1 %), iso-C16:0 (20.3 %), C16:0 (9.4 %) and anteiso-C17:0 (8.3 %). The G+C content of the genomic DNA was 71.1 %. Based on the polyphasic experiment results, the strain MBRL 179(T) merits recognition as a representative of a novel species of the genus Streptomyces for which the name Streptomyces muensis sp. nov. is proposed; the type strain is MBRL 179(T) (=JCM 17576(T) = KCTC 29124(T)).
一株从印度曼尼普尔邦 Hundung 的石灰岩采石场采集的样品中分离得到的链霉菌 MBRL 179(T)，经过多相分类学研究。该菌株与棘孢小单孢菌 NBRC 14228(T)（16S rRNA 基因序列相似性为 99.3%）在系统发育树中形成一个单系分支。DNA-DNA 杂交实验表明 MBRL 179(T)与棘孢小单孢菌 NBRC 14228(T)的 DNA-DNA 同源性为 34.7%。MBRL 179(T)菌株的全细胞水解物中含有 LL-二氨基庚二酸、木糖、葡萄糖和甘露糖，以及少量的核糖。检测到的主要极性脂类有双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇和磷脂酰肌醇甘露糖苷，以及其他未知的磷脂和氨基磷脂。主要的menaquinones 为 MK-9(H6)、MK-9(H8)和 MK-9(H4)。主要脂肪酸为anteiso-C16:0（28.1%）、iso-C16:0（20.3%）、C16:0（9.4%）和anteiso-C17:0（8.3%）。基因组 DNA 的 G+C 含量为 71.1%。基于多相实验结果，MBRL 179(T)菌株值得被认定为链霉菌属的一个新种代表，命名为Streptomyces muensis sp. nov.；其模式菌株为 MBRL 179(T)（=JCM 17576(T) = KCTC 29124(T)）。
