Piriyapongsa Jittima, Bootchai Chaiwat, Ngamphiw Chumpol, Tongsima Sissades
Genome Technology Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand
Genome Technology Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand.
Database (Oxford). 2014 Nov 25;2014:bau115. doi: 10.1093/database/bau115. Print 2014.
microRNA (miRNA)-promoter interaction resource (microPIR) is a public database containing over 15 million predicted miRNA target sites located within human promoter sequences. These predicted targets are presented along with their related genomic and experimental data, making the microPIR database the most comprehensive repository of miRNA promoter target sites. Here, we describe major updates of the microPIR database including new target predictions in the mouse genome and revised human target predictions. The updated database (microPIR2) now provides ∼80 million human and 40 million mouse predicted target sites. In addition to being a reference database, microPIR2 is a tool for comparative analysis of target sites on the promoters of human-mouse orthologous genes. In particular, this new feature was designed to identify potential miRNA-promoter interactions conserved between species that could be stronger candidates for further experimental validation. We also incorporated additional supporting information to microPIR2 such as nuclear and cytoplasmic localization of miRNAs and miRNA-disease association. Extra search features were also implemented to enable various investigations of targets of interest. Database URL: http://www4a.biotec.or.th/micropir2
微小RNA（miRNA）-启动子相互作用资源库（microPIR）是一个公共数据库，包含超过1500万个位于人类启动子序列内的预测miRNA靶位点。这些预测的靶标与其相关的基因组和实验数据一同呈现，使得microPIR数据库成为miRNA启动子靶位点最全面的存储库。在此，我们描述了microPIR数据库的主要更新内容，包括小鼠基因组中的新靶标预测和修订后的人类靶标预测。更新后的数据库（microPIR2）现在提供约8000万个人类和4000万个小鼠预测靶位点。除作为参考数据库外，microPIR2还是用于比较分析人鼠直系同源基因启动子上靶位点的工具。特别是，这一新功能旨在识别物种间保守的潜在miRNA-启动子相互作用，这些相互作用可能是进一步实验验证的更强候选对象。我们还将其他支持信息纳入了microPIR2，如miRNA的核定位和胞质定位以及miRNA与疾病的关联。还实施了额外的搜索功能，以便对感兴趣的靶标进行各种研究。数据库网址：http://www4a.biotec.or.th/micropir2
