Masahiro Otsu, Department of Chemistry, Kyorin University School of Medicine, Shinkawa, Mitaka , Tokyo 181-8611, Japan.
World J Stem Cells. 2014 Nov 26;6(5):651-7. doi: 10.4252/wjsc.v6.i5.651.
Basic research on pluripotent stem cells is designed to enhance understanding of embryogenesis, whereas applied research is designed to develop novel therapies and prevent diseases. Attainment of these goals has been enhanced by the establishment of embryonic stem cell lines, the technological development of genomic reprogramming to generate induced-pluripotent stem cells, and improvements in vitro techniques to manipulate stem cells. This review summarizes the techniques required to generate neural cells from pluripotent stem cells. In particular, this review describes current research applications of a simple neural differentiation method, the neural stem sphere method, which we developed.
多能干细胞的基础研究旨在增强对胚胎发生的理解，而应用研究旨在开发新的治疗方法和预防疾病。通过建立胚胎干细胞系、基因组重编程技术来产生诱导多能干细胞，以及改进体外技术来操纵干细胞，这些目标得到了加强。本综述总结了从多能干细胞中生成神经细胞所需的技术。特别是，本综述描述了我们开发的一种简单的神经分化方法——神经球方法的当前研究应用。
