Artiukhov V G, Kovaleva T A, Makarova E L, Kozhokina O M
Radiats Biol Radioecol. 2013 Jul-Aug;53(4):394-400.
The effect of UV light (240-390 nm) at the doses of 151-1510 J/m2 on the catalytic activity of free and im- mobilized glucoamylase from Aspergillus awamori was investigated. It was established that the loss of catalytic activity of the enzyme was associated with photochemical transformations of Trp-120, which is part of the active site of glucoamylase. It was shown that the use of collagen as a carrier for immobilization of glucoamylase reduces the constant photoinactivation of the enzyme. It was suggested that the protective effect of collagen in relation to the glucoamylase subjected to UV irradiation may be related to the acceptance of reactive oxygen species by collagen and the formation of the complex carrier-enzyme which is more photostable than the native enzyme.
研究了剂量为151 - 1510 J/m²的紫外线（240 - 390 nm）对泡盛曲霉游离和固定化葡糖淀粉酶催化活性的影响。已确定该酶催化活性的丧失与色氨酸-120的光化学转化有关，色氨酸-120是葡糖淀粉酶活性位点的一部分。结果表明，使用胶原蛋白作为固定葡糖淀粉酶的载体可降低该酶的持续光失活。有人提出，胶原蛋白对受紫外线照射的葡糖淀粉酶的保护作用可能与胶原蛋白对活性氧的接受以及形成比天然酶更耐光的载体-酶复合物有关。
