Nan Xiaoning, Wei Cong, He Hong
a College of Forestry, Northwest A&F University , Yangling , P.R. China and.
b College of Plant Protection, Northwest A&F University , Yangling , P.R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 May;27(3):2060-1. doi: 10.3109/19401736.2014.982556. Epub 2014 Nov 27.
The complete mitochondrial genome of Eucryptorrhynchus brandti (Harold) (Insecta: Coleoptera: Curculionidae) were reconstructed from whole-genome Illumina Hiseq 2000 sequencing data with an average coverage of 1406.7X. The circular genome is 15,122 bp in length, consisting of 13 protein-coding genes (PCGs), 21 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs) and one D-loop or control region. The tRNA-Ile gene was not found in the mitochondrial genome, as is identical to two other curculionidae species, i.e. Sphenophorus sp. (GU176342) and Naupactus xanthographus (GU176345). All PCGs initiated with ATN codons, except for the ND1 started with TTG. Two PCGs (COI and ND4) have an incomplete stop codon T. Two PCGs (ND4L and ND1) harbor the stop codon TAG, while all other PCGs terminated with the TAA codon. The nucleotide composition is highly asymmetric (38.7% A, 14.4% C, 9.2% G and 37.8% T) with an overall AT content of 76.5%.
利用平均覆盖度为1406.7X的全基因组Illumina Hiseq 2000测序数据，重建了黄斑齿爪鳃金龟（Eucryptorrhynchus brandti (Harold)）（昆虫纲：鞘翅目：象甲科）的完整线粒体基因组。该环状基因组长度为15,122 bp，由13个蛋白质编码基因（PCGs）、21个转运RNA（tRNAs）、两个核糖体RNA（rRNAs）和一个D环或控制区组成。线粒体基因组中未发现tRNA-Ile基因，这与另外两种象甲科物种，即斯氏象（Sphenophorus sp.）（GU176342）和黄斑长跗萤叶甲（Naupactus xanthographus）（GU176345）相同。所有PCGs均以ATN密码子起始，但ND1以TTG起始。两个PCGs（COI和ND4）具有不完全终止密码子T。两个PCGs（ND4L和ND1）含有终止密码子TAG，而所有其他PCGs以TAA密码子终止。核苷酸组成高度不对称（A占38.7%，C占14.4%，G占9.2%，T占37.8%），总体AT含量为76.5%。
