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(鞘翅目:象甲科)的完整线粒体基因组。

The complete mitochondrial genome of (Coleoptera: Curculionidae).

作者信息

Zhang Feng, Hong Bo, Chen Zhi-Jie, Wang Yuan-Zheng, Li Ying-Mei, Zhang Shu-Lian

机构信息

Bio-Agriculture Institute of Shaanxi, Xi'an, China.

出版信息

Mitochondrial DNA B Resour. 2017 Oct 17;2(2):718-719. doi: 10.1080/23802359.2017.1390413.

Abstract

The complete mitochondrial genome sequence of (Coleoptera: Curculionidae) was determined by using an Illumina platform. The circular genome was 16,472 bp in length and contained 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), 13 protein-coding genes (PCGs), and one control region. The nucleotide composition was significantly biased (A, G, C, and T was 39.74%, 10.11%, 15.41%, and 34.74%, respectively) with A + T contents of 74.49%. All PCGs were initiated with standard ATN (ATG/ATT) codons. While 10 PCGs were terminated with TAA, two PCGs were terminated with TAG ( and ), and was terminated with an incomplete stop codon TA. All tRNAs were predicted to contain typical cloverleaf secondary structures except . The phylogenetic analysis of the concatenated nucleotide sequences of 13 PCGs from 12 Curculionidae species was performed by using MrBayes 3.1.2. The results indicated that was more closely related to than to other species.

摘要

利用Illumina平台测定了(鞘翅目:象甲科)的完整线粒体基因组序列。该环状基因组长度为16,472 bp,包含22个转运RNA基因(tRNAs)、两个核糖体RNA基因(rRNAs)、13个蛋白质编码基因(PCGs)和一个控制区。核苷酸组成存在显著偏差(A、G、C和T分别为39.74%、10.11%、15.41%和34.74%),A+T含量为74.49%。所有PCGs均以标准的ATN(ATG/ATT)密码子起始。10个PCGs以TAA终止,两个PCGs以TAG(和)终止,以不完整的终止密码子TA终止。除了,所有tRNAs预计都含有典型的三叶草二级结构。利用MrBayes 3.1.2对12种象甲科物种的13个PCGs的串联核苷酸序列进行了系统发育分析。结果表明,与相比,与其他物种的关系更为密切。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6195/7799709/c085065027d3/TMDN_A_1390413_F0001_B.jpg

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