Hamza Samir, Bouchemi Meryem, Slimane Noureddine, Azari Zitouni
Biomaterials and Biomechanics Laboratory, National Institute M.T. Kassab of Orthopedic, 2010 La Manouba, Tunis, Tunisia.
National Institute of Applied Sciences and Technology, Centre Urbain Nord, Box 676, 1080 Tunis cedex, Tunisia.
Mater Sci Eng C Mater Biol Appl. 2013 Jan 1;33(1):537-42. doi: 10.1016/j.msec.2012.09.028. Epub 2012 Oct 6.
Bone substitutes are more and more used in bone surgery because of their biologic safety, clinic efficiency and facility to synthesize. Bone substitutes with active osteogenic properties, associating biomaterials with organic macromolecule components of the extracellular matrix (protein, GAG) are recommended. Nevertheless, we should have a simple technique to control interactions between proteins and the material. Natural coral and nacre have been found to be impressive bone graft substitutes. In this work, we characterize nacre and coral powder using energy dispersive X-ray analysis (EDX). We used electrochemical impedance spectroscopy (EIS) and attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy to evaluate bovine serum albumin (BSA) as model protein, adsorbed to these biomaterial surfaces. In order to understand the nacre/coral-protein interfacial compatibility, it is necessary to investigate the wettability.
由于其生物安全性、临床有效性和合成便利性，骨替代物在骨外科手术中的应用越来越广泛。推荐使用具有活性成骨特性的骨替代物，即将生物材料与细胞外基质的有机大分子成分（蛋白质、糖胺聚糖）相结合。然而，我们需要一种简单的技术来控制蛋白质与材料之间的相互作用。天然珊瑚和珍珠母已被证明是令人印象深刻的骨移植替代物。在这项工作中，我们使用能量色散X射线分析（EDX）对珍珠母和珊瑚粉末进行表征。我们使用电化学阻抗谱（EIS）和衰减全反射傅里叶变换红外（ATR-FTIR）光谱来评估吸附在这些生物材料表面的作为模型蛋白的牛血清白蛋白（BSA）。为了了解珍珠母/珊瑚-蛋白质界面的相容性，有必要研究其润湿性。
