Windhorst U, Laouris Y, Kokkoroyiannis T, Kuipers U, Meyer-Lohmann J
Universität Göttingen, Abteilung Neuro- und Sinnesphysiologie, Federal Republic of Germany.
Pflugers Arch. 1989 Apr;413(6):622-8. doi: 10.1007/BF00581812.
We here present a method to study the interaction of parallel neural input channels regarding their effects on a neurone. In particular, the method allows to disclose the effects of oligosynaptic pathways that may exist in parallel to direct monosynaptic connections to the cell. Two (or more) inputs (nerves) are stimulated with random patterns of stimuli. The response of the cell to these patterns is evaluated by the computation of peristimulus-time histograms (PSTHs). One of the two stimulus trains is selected as the one to yield reference events for the PSTH computation. From this stimulus train are selected those stimuli as reference events which are preceded, at defined mean intervals, by stimuli in the same or a parallel channel. These "conditioning" stimuli are determined (1) separately from each single stimulus train and (2) concomitantly from the two trains as events occurring simultaneously in both. The effects exerted by these various conditioning events on the effects of the "test" pulses on the cell response yield insights into the interactions between the two (or more) inputs. These methods are demonstrated on spinal Renshaw cells activated by independent random stimulation of two muscle nerves and on dorsal horn neurones responding to cutaneous nerve stimulation.
我们在此提出一种方法，用于研究平行神经输入通道之间的相互作用及其对神经元的影响。具体而言，该方法能够揭示可能与细胞的直接单突触连接并行存在的寡突触通路的影响。用随机刺激模式刺激两个（或更多）输入（神经）。通过计算刺激时间直方图（PSTH）来评估细胞对这些模式的反应。在两个刺激序列中选择其中一个作为产生用于PSTH计算的参考事件的序列。从该刺激序列中选择那些在相同或平行通道中的刺激以定义的平均间隔之前出现的刺激作为参考事件。这些“条件”刺激是（1）从每个单独的刺激序列中分别确定的，以及（2）从两个序列中同时确定为在两者中同时发生的事件。这些各种条件事件对“测试”脉冲对细胞反应的影响所产生的作用，有助于深入了解两个（或更多）输入之间的相互作用。这些方法在通过独立随机刺激两条肌肉神经激活的脊髓闰绍细胞以及对皮肤神经刺激作出反应的背角神经元上得到了验证。
