Liu Lei, Li Chu, Ma Jian, Wu Yingdong, Ni Zhonghua, Chen Yunfei
Suzhou Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Suzhou Research Institute of Southeast University, Suzhou 215123, People's Republic of China.
Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing, Jiangsu 211189, People's Republic of China.
IET Nanobiotechnol. 2014 Dec;8(4):247-56. doi: 10.1049/iet-nbt.2013.0017.
Novel generation of analytical technology based on nanopores has provided possibilities to fabricate nanofluidic devices for low-cost DNA sequencing or rapid biosensing. In this paper, a simplified model was suggested to describe DNA molecule's translocation through a nanopore, and the internal potential, ion concentration, ionic flowing speed and ionic current in nanopores with different sizes were theoretically calculated and discussed on the basis of Poisson-Boltzmann equation, Navier-Stokes equation and Nernst-Planck equation by considering several important parameters, such as the applied voltage, the thickness and the electric potential distributions in nanopores. In this way, the basic ionic currents, the modulated ionic currents and the current drops induced by translocation were obtained, and the size effects of the nanopores were carefully compared and discussed based on the calculated results and experimental data, which indicated that nanopores with a size of 10 nm or so are more advantageous to achieve high quality ionic current signals in DNA sensing.
基于纳米孔的新一代分析技术为制造用于低成本DNA测序或快速生物传感的纳米流体装置提供了可能性。本文提出了一个简化模型来描述DNA分子通过纳米孔的转运，并基于泊松-玻尔兹曼方程、纳维-斯托克斯方程和能斯特-普朗克方程，通过考虑几个重要参数，如施加电压、纳米孔的厚度和电势分布，对不同尺寸纳米孔内的内部电势、离子浓度、离子流动速度和离子电流进行了理论计算和讨论。通过这种方式，获得了基本离子电流、调制离子电流和转运引起的电流下降，并根据计算结果和实验数据仔细比较和讨论了纳米孔的尺寸效应，结果表明尺寸约为10 nm的纳米孔在DNA传感中更有利于获得高质量的离子电流信号。
