β-肾上腺素能受体激活对星形细胞瘤细胞中神经生长因子生物合成的调节：c-Fos蛋白的潜在作用。

Regulation of nerve growth factor biosynthesis by beta-adrenergic receptor activation in astrocytoma cells: a potential role of c-Fos protein.

作者信息

Mocchetti I, De Bernardi M A, Szekely A M, Alho H, Brooker G, Costa E

机构信息

Fidia-Georgetown Institute for the Neurosciences, Georgetown University, School of Medicine, Washington, DC 20007.

出版信息

Proc Natl Acad Sci U S A. 1989 May;86(10):3891-5. doi: 10.1073/pnas.86.10.3891.

The chain of events that results in increased production of nerve growth factor (NGF) following beta-adrenergic receptor (BAR) stimulation has been investigated in the C6-2B rat astrocytoma cell line. Exposure of these cells to the BAR agonist isoproterenol elicits the following cascade of events: (i) increase of cAMP content; (ii) increase of c-Fos mRNA content; (iii) accumulation of c-Fos protein immunoreactivity in the nucleus; (iv) increase of NGF mRNA content. The increase in c-Fos mRNA and its translation product are early events (15 and 40 min, respectively) and precede the accumulation of NGF mRNA, which peaks at 3 hr. The increase in the two mRNAs appears interrelated because cycloheximide inhibits the accumulation of c-Fos protein and NGF mRNA elicited by isoproterenol. Moreover, the accumulation of nuclear c-Fos protein and NGF mRNA induced by BAR stimulation is reduced by 2-aminopurine, an inhibitor of c-Fos mRNA induction. These data suggest that, in C6-2B astrocytoma cells, the nuclear accumulation of c-Fos protein is required for the induction of NGF mRNA expression by BAR stimulation.

在C6 - 2B大鼠星形细胞瘤细胞系中，研究了β - 肾上腺素能受体（BAR）刺激后导致神经生长因子（NGF）产量增加的一系列事件。将这些细胞暴露于BAR激动剂异丙肾上腺素会引发以下一系列事件：（i）cAMP含量增加；（ii）c - Fos mRNA含量增加；（iii）c - Fos蛋白免疫反应性在细胞核中积累；（iv）NGF mRNA含量增加。c - Fos mRNA及其翻译产物的增加是早期事件（分别为15分钟和40分钟），且先于NGF mRNA的积累，NGF mRNA在3小时达到峰值。两种mRNA的增加似乎相互关联，因为环己酰亚胺抑制了异丙肾上腺素引起的c - Fos蛋白和NGF mRNA的积累。此外，BAR刺激诱导的细胞核c - Fos蛋白和NGF mRNA的积累被2 - 氨基嘌呤（一种c - Fos mRNA诱导抑制剂）减少。这些数据表明，在C6 - 2B星形细胞瘤细胞中，BAR刺激诱导NGF mRNA表达需要c - Fos蛋白在细胞核中积累。