Tsukada T, Horovitch S J, Montminy M R, Mandel G, Goodman R H
DNA. 1985 Aug;4(4):293-300. doi: 10.1089/dna.1985.4.293.
Vasoactive intestinal polypeptide (VIP) is a 28-amino-acid hormone produced primarily by neural tissues. The amino acid sequence of VIP is similar to that of a number of gastrointestinal hormones, including glucagon and secretin. VIP is synthesized as part of a polyprotein, pre-proVIP, which generates, in addition to VIP, an additional bioactive peptide known as PHM. As a first step toward understanding the molecular basis of pre-proVIP gene expression, we have isolated the pre-proVIP gene and have determined its structure. The gene is approximately 9 kb long and is interrupted by six introns which appear to divide the gene into functional domains. One of the introns occurs within the 3'-untranslated region of the gene.
血管活性肠肽（VIP）是一种主要由神经组织产生的含28个氨基酸的激素。VIP的氨基酸序列与许多胃肠激素相似，包括胰高血糖素和促胰液素。VIP作为一种多蛋白前体（前-前体VIP）的一部分被合成，除了VIP之外，还产生一种名为PHM的额外生物活性肽。作为理解前-前体VIP基因表达分子基础的第一步，我们分离了前-前体VIP基因并确定了其结构。该基因大约9千碱基对长，被六个内含子打断，这些内含子似乎将基因分成功能域。其中一个内含子出现在基因的3'非翻译区内。
