Dufresnes Christophe, Brelsford Alan, Perrin Nicolas
Department of Ecology and Evolution, Biophore building, University of Lausanne, 1015 Lausanne, Switzerland.
BMC Res Notes. 2014 Nov 26;7:850. doi: 10.1186/1756-0500-7-850.
Western Palearctic tree frogs (Hyla arborea group) represent a strong potential for evolutionary and conservation genetic research, so far underexploited due to limited molecular resources. New microsatellite markers have recently been developed for Hyla arborea, with high cross-species utility across the entire circum-Mediterranean radiation. Here we conduct sibship analyses to map available markers for use in future population genetic applications.
We characterized eight linkage groups, including one sex-linked, all showing drastically reduced recombination in males compared to females, as previously documented in this species. Mapping of the new 15 markers to the ~200 My diverged Xenopus tropicalis genome suggests a generally conserved synteny with only one confirmed major chromosome rearrangement.
The new microsatellites are representative of several chromosomes of H. arborea that are likely to be conserved across closely-related species. Our linkage map provides an important resource for genetic research in European Hylids, notably for studies of speciation, genome evolution and conservation.
西古北界树蛙（雨蛙属）在进化和保护遗传学研究方面具有巨大潜力，但由于分子资源有限，目前尚未得到充分利用。最近已为绿雨蛙开发了新的微卫星标记，这些标记在整个环地中海辐射区域具有较高的跨物种通用性。在此，我们进行同胞关系分析，以绘制可用于未来种群遗传学应用的标记图谱。
我们鉴定出八个连锁群，其中包括一个性连锁群，所有连锁群均显示雄性的重组率相比雌性大幅降低，这与该物种先前的记录一致。将新的15个标记定位到约2亿年前分化的热带爪蟾基因组上，结果表明其染色体同线性总体保守，仅确认有一次主要的染色体重排。
新的微卫星代表了绿雨蛙的几条染色体，这些染色体可能在近缘物种中保守。我们的连锁图谱为欧洲雨蛙科的遗传学研究提供了重要资源，特别是对于物种形成、基因组进化和保护研究。
