Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, Hubei, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
BMC Genomics. 2021 Apr 1;22(1):230. doi: 10.1186/s12864-021-07550-0.
The Chinese giant salamander Andrias davidianus is an important amphibian species in China because of its increasing economic value, protection status and special evolutionary position from aquatic to terrestrial animal. Its large genome presents challenges to genetic research. Genetic linkage mapping is an important tool for genome assembly and determination of phenotype-related loci.
In this study, we constructed a high-density genetic linkage map using ddRAD sequencing technology to obtain SNP genotyping data of members from an full-sib family which sex had been determined. A total of 10,896 markers were grouped and oriented into 30 linkage groups, representing 30 chromosomes of A. davidianus. The genetic length of LGs ranged from 17.61 cM (LG30) to 280.81 cM (LG1), with a mean inter-locus distance ranging from 0.11(LG3) to 0.48 cM (LG26). The total genetic map length was 2643.10 cM with an average inter-locus distance of 0.24 cM. Three sex-related loci and four sex-related markers were found on LG6 and LG23, respectively.
We constructed the first High-density genetic linkage map and identified three sex-related loci in the Chinese giant salamander. Current results are expected to be a useful tool for future genomic studies aiming at the marker-assisted breeding of the species.
中国大鲵（Andrias davidianus）是中国重要的两栖动物物种，具有日益增长的经济价值、保护地位以及从水生到陆生动物的特殊进化地位。其庞大的基因组对遗传研究提出了挑战。遗传连锁图谱构建是基因组组装和确定表型相关基因座的重要工具。
本研究利用 ddRAD 测序技术构建了高密度遗传连锁图谱，获得了已确定性别的全同胞家系成员的 SNP 基因型数据。共将 10896 个标记分组并定向到 30 个连锁群中，代表中国大鲵的 30 条染色体。LGs 的遗传长度范围从 17.61cM（LG30）到 280.81cM（LG1），基因座间平均距离范围从 0.11cM（LG3）到 0.48cM（LG26）。总遗传图谱长度为 2643.10cM，基因座间平均距离为 0.24cM。在 LG6 和 LG23 上分别发现了三个与性别相关的基因座和四个与性别相关的标记。
本研究构建了中国大鲵的第一张高密度遗传连锁图谱，并鉴定了三个与性别相关的基因座。目前的结果有望成为该物种标记辅助育种等未来基因组研究的有用工具。
