Reverté Laia, Soliño Lucía, Carnicer Olga, Diogène Jorge, Campàs Mònica
IRTA, Carretera Poble Nou km 5.5, 43540 Sant Carles de la Ràpita, Spain.
Mar Drugs. 2014 Nov 26;12(12):5719-63. doi: 10.3390/md12125719.
The emergence of marine toxins in water and seafood may have a considerable impact on public health. Although the tendency in Europe is to consolidate, when possible, official reference methods based on instrumental analysis, the development of alternative or complementary methods providing functional or toxicological information may provide advantages in terms of risk identification, but also low cost, simplicity, ease of use and high-throughput analysis. This article gives an overview of the immunoassays, cell-based assays, receptor-binding assays and biosensors that have been developed for the screening and quantification of emerging marine toxins: palytoxins, ciguatoxins, cyclic imines and tetrodotoxins. Their advantages and limitations are discussed, as well as their possible integration in research and monitoring programs.
水和海产品中海洋毒素的出现可能会对公众健康产生重大影响。尽管在欧洲，趋势是尽可能整合基于仪器分析的官方参考方法，但开发能够提供功能或毒理学信息的替代或补充方法，在风险识别方面可能具有优势，而且成本低、操作简单、易于使用且能进行高通量分析。本文概述了已开发用于新兴海洋毒素（如岩沙海葵毒素、雪卡毒素、环亚胺和河豚毒素）筛选和定量的免疫测定法、基于细胞的测定法、受体结合测定法和生物传感器。讨论了它们的优点和局限性，以及它们在研究和监测计划中可能的整合情况。
