Takemori Nobuaki, Takemori Ayako, Matsuoka Kazuhiro, Morishita Ryo, Matsushita Natsuki, Aoshima Masato, Takeda Hiroyuki, Sawasaki Tatsuya, Endo Yaeta, Higashiyama Shigeki
Proteo-Science Center, Ehime University, Ehime, Japan.
Mol Biosyst. 2015 Feb;11(2):361-5. doi: 10.1039/c4mb00556b. Epub 2014 Nov 28.
Using a wheat germ cell-free protein synthesis system, we developed a high-throughput method for the synthesis of stable isotope-labeled full-length transmembrane proteins as proteoliposomes to mimic the in vivo environment, and we successfully constructed an internal standard library for targeted transmembrane proteomics by using mass spectrometry.
利用小麦胚无细胞蛋白质合成系统，我们开发了一种高通量方法，用于合成稳定同位素标记的全长跨膜蛋白作为蛋白脂质体，以模拟体内环境，并且我们通过质谱成功构建了用于靶向跨膜蛋白质组学的内标库。
