新型苏云金芽孢杆菌分离株对埃及伊蚊（双翅目：蚊科）和地中海实蝇（双翅目：实蝇科）的特性研究

Characterisation of novel Bacillus thuringiensis isolates against Aedes aegypti (Diptera: Culicidae) and Ceratitis capitata (Diptera: Tephridae).

作者信息

Elleuch Jihen, Tounsi Slim, Ben Hassen Najeh Belguith, Lacoix Marie Noël, Chandre Fabrice, Jaoua Samir, Zghal Raida Zribi

机构信息

Biopesticides Team [L.P.I.P.], Center of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia.

Institut de Recherche pour le Développement (IRD), UMR MIVEGEC (UM1-UM2-CNRS 5290-IRD 224), Maladies Infectieuses et Vecteurs, Ecologie, Génétique, Evolution et Contrôle, Laboratoire de Lutte contre les Insectes Nuisibles (LIN), Montpellier, France.

出版信息

J Invertebr Pathol. 2015 Jan;124:90-7. doi: 10.1016/j.jip.2014.11.005. Epub 2014 Nov 26.

DOI:10.1016/j.jip.2014.11.005

PMID:25433312

Abstract

Bacillus thuringiensis is successfully used in pest management strategies as an eco-friendly bioinsecticide. Isolation and identification of new strains with a wide variety of target pests is an ever growing field. In this paper, new B. thuringiensis isolates were investigated to search for original strains active against diptera and able to produce novel toxins that could be used as an alternative for the commercial H14 strain. Biochemical and molecular characterization revealed a remarkable diversity among the studied strains. Using the PCR method, cry4C/Da1, cry30Ea, cry39A, cry40 and cry54 genes were detected in four isolates. Three strains, BLB355, BLB196 and BUPM109, showed feeble activities against Aedes aegypti larvae. Interestingly, spore-crystal mixtures of BLB361, BLB30 and BLB237 were found to be active against Ceratitis capitata with an LC50 value of about 65.375, 51.735 and 42.972 μg cm(-2), respectively. All the studied strains exhibited important mortality levels using culture supernatants against C. capitata larvae. This suggests that these strains produce a wide range of soluble factors active against C. capitata larvae.

摘要

苏云金芽孢杆菌作为一种生态友好型生物杀虫剂，已成功应用于害虫管理策略中。分离和鉴定针对多种目标害虫的新菌株是一个不断发展的领域。本文对新的苏云金芽孢杆菌分离株进行了研究，以寻找对双翅目有活性且能够产生新型毒素的原始菌株，这些新型毒素可作为商业H14菌株的替代品。生化和分子特征分析表明，所研究的菌株之间存在显著差异。采用PCR方法，在4株分离株中检测到了cry4C/Da1、cry30Ea、cry39A、cry40和cry54基因。三株菌株BLB355、BLB196和BUPM109对埃及伊蚊幼虫表现出微弱的活性。有趣的是，发现BLB361、BLB30和BLB237的芽孢-晶体混合物对地中海实蝇有活性，其LC50值分别约为65.375、51.735和42.972μg cm(-2)。所有研究菌株使用培养上清液处理地中海实蝇幼虫时均表现出较高的死亡率。这表明这些菌株产生了多种对地中海实蝇幼虫有活性的可溶性因子。