Rugolo M, Mastocola T, Flamigni A, Lenaz G
Dipartimento di Biologia Evoluzionistica e Sperimentale, Universita' di Bologna, Italy.
Biochem Biophys Res Commun. 1989 May 15;160(3):1330-8. doi: 10.1016/s0006-291x(89)80149-4.
Incubation of human skin fibroblasts in hypotonic media induced the activation of 36Cl- efflux which was roughly proportional to the decrease in the osmolality of the media. The efflux of 36Cl- was insensitive to DIDS plus furosemide and inhibited by addition of a Cl- channel blocker such as 5-nitro-2-(3-phenyl propylamino) benzoic acid (NPPB). We propose that a conductive pathway for Cl- transport, almost silent in isotonic conditions, is activated by exposing human fibroblasts to hypotonic shock, this conclusion being supported by evidence that also 36Cl- influx was enhanced by hypotonic medium.
