Dissing S, Hoffman R, Murnane M J, Hoffman J F
Am J Physiol. 1984 Jul;247(1 Pt 1):C53-60. doi: 10.1152/ajpcell.1984.247.1.C53.
The Cl- transport characteristics of the human leukemic cell lines K562 and HL60, with erythroid and granulocytic phenotypic features, respectively, were investigated. Cl- effluxes were measured with 36Cl- under equilibrium conditions in both cell lines and were found to be three orders of magnitude slower than the unidirectional efflux of Cl- in normal erythrocytes. Induction of differentiation of the K562 cell line with hemin does not affect the rate of Cl- transport, while induction of the HL60 cell line with dimethyl sulfoxide results in a small decrease in the rate of Cl- transport. Cl- transport in both cell lines could be divided into two components. One component is inhibited by treatment with 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), displays counter-transport characteristics, and has a high energy of activation--all properties characteristic of the human erythrocyte-facilitated anion exchange system. The second component is insensitive to DIDS, is partially inhibited by furosemide, and has a low energy of activation.
分别研究了具有红系和粒系表型特征的人白血病细胞系K562和HL60的氯离子转运特性。在平衡条件下用³⁶Cl⁻测量了这两种细胞系的氯离子外流，发现其比正常红细胞中氯离子的单向外流慢三个数量级。用血红素诱导K562细胞系分化不影响氯离子转运速率，而用二甲亚砜诱导HL60细胞系分化会导致氯离子转运速率略有下降。两种细胞系中的氯离子转运可分为两个部分。一部分在用4,4'-二异硫氰基芪-2,2'-二磺酸（DIDS）处理时受到抑制，表现出反向转运特性，并且具有较高的活化能——这些都是人红细胞促进性阴离子交换系统的特征性质。第二部分对DIDS不敏感，部分受到呋塞米抑制，并且具有较低的活化能。
