磷脂酶Cε1（PLCE1）单倍型与克什米尔山谷地区胃癌风险增加相关。
Phospholipase C epsilon 1 (PLCE1) haplotypes are associated with increased risk of gastric cancer in Kashmir Valley.
作者信息
Malik Manzoor A, Srivastava Priya, Zargar Showkat A, Mittal Balraj
机构信息
Department of Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareilly Road, Lucknow, Uttar Pradesh, India.
出版信息
Saudi J Gastroenterol. 2014 Nov-Dec;20(6):371-7. doi: 10.4103/1319-3767.145330.
BACKGROUND/AIM: Phospholipase C epsilon 1 (PLCE1) plays a crucial role in carcinogenesis and progression of several types of cancers. A single nucleotide polymorphism (SNP, rs2274223) in PLCE1 has been identified as a novel susceptibility locus. The aim of the present study was to investigate the role of three potentially functional SNPs (rs2274223A > G, rs3765524C > T, and rs7922612C > T) of PLCE1 in gastric cancer patients from Kashmir Valley.
PATIENTS AND METHODS
The study was conducted in 108 GC cases and 195 healthy controls from Kashmir Valley. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism method. Data were statistically analyzed using c2 test and logistic regression models. A P value of less than 0.05 was regarded as statistically significant.
RESULTS
The frequency of PLCE1 A2274223C3765524T7922612, G2274223C3765524T7922612 , and G2274223T3765524C7922612 haplotypes were higher in patients compared with controls, conferred high risk for GC [odds ratio (OR) =6.29; P = 0.001; Pcorr = 0.003], (OR = 3.23; P = 0.011; Pcorr = 0.033), and (OR = 5.14; P = 0.011; Pcorr = 0.033), respectively. Smoking and salted tea are independent risk factors for GC, but we did not find any significant modulation of cancer risk by PLCE1 variants with smoking or excessive consumption of salted tea.
CONCLUSION
These results suggest that variation in PLCE1 may be associated with GC risk in Kashmir Valley.
背景/目的：磷脂酶Cε1（PLCE1）在多种癌症的发生和发展中起关键作用。PLCE1中的一个单核苷酸多态性（SNP，rs2274223）已被确定为一个新的易感位点。本研究的目的是调查PLCE1的三个潜在功能性SNP（rs2274223A>G、rs3765524C>T和rs7922612C>T）在克什米尔山谷胃癌患者中的作用。
患者与方法
该研究在克什米尔山谷的108例胃癌病例和195名健康对照者中进行。采用聚合酶链反应-限制性片段长度多态性方法进行基因分型。使用卡方检验和逻辑回归模型对数据进行统计学分析。P值小于0.05被认为具有统计学意义。
结果
与对照组相比，患者中PLCE1 A2274223C3765524T7922612、G2274223C3765524T7922612和G2274223T3765524C7922612单倍型的频率更高，赋予了较高的胃癌风险[比值比（OR）=6.29；P=0.001；校正P=0.003]，（OR=3.23；P=0.011；校正P=0.033），以及（OR=5.14；P=0.011；校正P=0.033）。吸烟和咸茶是胃癌的独立危险因素，但我们未发现PLCE1变异与吸烟或过量饮用咸茶对癌症风险有任何显著的调节作用。
结论
这些结果表明，PLCE1的变异可能与克什米尔山谷的胃癌风险相关。
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