Waheed M M, Ghoneim I M, Alhaider A K
Department of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hufof, Kingdom of Saudi Arabia; Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Department of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hufof, Kingdom of Saudi Arabia; Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Theriogenology. 2015 Mar 1;83(4):650-4. doi: 10.1016/j.theriogenology.2014.10.033. Epub 2014 Nov 10.
Eight healthy fertile (control) and 11 infertile male dromedaries were used to investigate whether specific seminal plasma and serum fertility biomarkers could be related to their in vivo fertility. Eight fertility biomarkers and testosterone were determined in both seminal plasma and serum of all studied camels during the rutting season using commercial kits. Results revealed a significant (P < 0.01) difference in semen parameters between the control and infertile camels. There was a significant (P < 0.01) difference between the control and infertile dromedaries in seminal plasma glutathione peroxidase (GPx) activity (15.04 ± 1.14 vs. 4.55 ± 0.96 nmol/min/mL, respectively) and both phospholipase A2 (sPLA2; 50.66 ± 6.28 vs. 23.56 ± 4.29 pg/mL, respectively) and testosterone concentrations (732.14 ± 57.12 vs. 396.36 ± 79.34 pg/mL, respectively). A significant (P < 0.05) difference was found between the control and infertile camels in seminal plasma concentrations of osteopontin, cysteine-rich secretory protein 3 (CRISP3), and prostaglandin D synthase (lipocalcin-type). There was a significant (P < 0.01) difference between the fertile and infertile camels in serum GPx activity 67.81 ± 12.41 vs. 21.31 ± 4.63 nmol/min/mL, respectively) and both testosterone (599.57 ± 110.90 vs. 176.09 ± 24.05 pg/mL, respectively) and clusterin concentrations (137.29 ± 14.15 vs. 253.00 ± 17.14 ng/mL, respectively). A significant (P < 0.05) difference existed between the control and infertile male dromedaries in serum concentrations of sPLA2, CRISP3, malonialdehyde, and insulinlike growth factor 1. In conclusion, CRISP3, sPLA2, GPx, and testosterone are fertility-associated biomarkers in both seminal plasma and serum of dromedary camels. Seminal plasma osteopontin is positively correlated and prostaglandin D synthase (lipocalcin-type) is negatively correlated with camels' fertility. Serum malonialdehyde, insulinlike growth factor 1, and clusterin are negatively correlated with fertility of male dromedary camels.
选取8头健康且具有生育能力的（对照）雄性单峰驼和11头不育雄性单峰驼，以研究特定的精浆和血清生育生物标志物是否与它们的体内生育能力相关。在发情季节，使用商用试剂盒测定了所有受试骆驼精浆和血清中的8种生育生物标志物及睾酮。结果显示，对照骆驼和不育骆驼的精液参数存在显著差异（P < 0.01）。对照单峰驼和不育单峰驼的精浆谷胱甘肽过氧化物酶（GPx）活性（分别为15.04 ± 1.14与4.55 ± 0.96 nmol/min/mL）、磷脂酶A2（sPLA2；分别为50.66 ± 6.28与23.56 ± 4.29 pg/mL）以及睾酮浓度（分别为732.14 ± 57.12与396.36 ± 79.34 pg/mL）均存在显著差异（P < 0.01）。对照骆驼和不育骆驼的精浆骨桥蛋白、富含半胱氨酸的分泌蛋白3（CRISP3）以及前列腺素D合酶（脂质运载蛋白型）浓度存在显著差异（P < 0.05）。可育骆驼和不育骆驼的血清GPx活性（分别为67.81 ± 12.41与21.31 ± 4.63 nmol/min/mL）、睾酮（分别为599.57 ± 110.90与176.09 ± 24.05 pg/mL）以及聚集素浓度（分别为137.29 ± 14.15与253.00 ± 17.14 ng/mL）均存在显著差异（P < 0.01）。对照雄性单峰驼和不育雄性单峰驼的血清sPLA2、CRISP3、丙二醛和胰岛素样生长因子1浓度存在显著差异（P < 0.05）。总之，CRISP3、sPLA2、GPx和睾酮是单峰驼精浆和血清中与生育相关的生物标志物。精浆骨桥蛋白与骆驼的生育能力呈正相关，而前列腺素D合酶（脂质运载蛋白型）与骆驼的生育能力呈负相关。血清丙二醛、胰岛素样生长因子1和聚集素与雄性单峰驼的生育能力呈负相关。
