Grupo BIOFITER (Biología, Fisiología y Tecnologías de la Reproducción), Departamento de Bioquímica y Biología Molecular y Celular, Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, Spain.
Departamento de Producción Animal, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogotá 111321, Colombia.
Int J Mol Sci. 2020 Oct 29;21(21):8063. doi: 10.3390/ijms21218063.
This work aimed to determine the contribution of the testis and epididymis and the effect of the photoperiodic regimen on ram seminal plasma (SP). Semen was collected from 15 mature rams located in an equatorial (Colombian Creole and Romney Marsh, eight intact and two vasectomized) or a temperate climate (Rasa Aragonesa, three intact and two vasectomized). SP proteins were analyzed by Bradford, SDS-PAGE and difference gel electrophoresis (DIGE). Melatonin and testosterone concentrations were quantified by ELISA, and activity of glutathione peroxidase (GPx), glutathione reductase (GRD), and catalase by enzymatic assays. Vasectomy increased protein concentration and the intensity of high molecular weight bands ( < 0.001), with no differences between breeds. DIGE revealed the absence of six proteins in vasectomized rams: angiotensin-converting enzyme, lactotransferrin, phosphoglycerate kinase, sorbitol dehydrogenase, epididymal secretory glutathione peroxidase and epididymal secretory protein E1. Vasectomy also decreased melatonin concentrations in seasonal rams, and testosterone in all of them ( < 0.001), but did not affect antioxidant enzyme activity. Equatorial rams showed lower melatonin and testosterone concentration ( < 0.01) and catalase, but higher GPx activity ( < 0.05). In conclusion, vasectomy modifies the protein profile and hormonal content of ram seminal plasma, whereas the exposure to a constant photoperiod affects hormonal concentration and antioxidant enzymes activity.
本研究旨在确定睾丸和附睾的作用以及光照周期对公羊精液（SP）的影响。从位于赤道气候（哥伦比亚克里奥尔语和罗姆尼马什，8 个完整和 2 个结扎）或温带气候（拉撒罗阿雷纳，3 个完整和 2 个结扎）的 15 只成熟公羊中采集精液。通过 Bradford、SDS-PAGE 和差异凝胶电泳（DIGE）分析 SP 蛋白。通过 ELISA 定量测定褪黑素和睾酮浓度，并通过酶促测定法测定谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GRD）和过氧化氢酶的活性。结扎增加了蛋白质浓度和高分子量带的强度（<0.001），不同品种之间没有差异。DIGE 显示结扎公羊中缺少 6 种蛋白质：血管紧张素转换酶、乳转铁蛋白、磷酸甘油酸激酶、山梨醇脱氢酶、附睾分泌谷胱甘肽过氧化物酶和附睾分泌蛋白 E1。结扎还降低了季节性公羊的褪黑素浓度和所有公羊的睾酮浓度（<0.001），但不影响抗氧化酶活性。赤道地区的公羊表现出较低的褪黑素和睾酮浓度（<0.01）和过氧化氢酶，但较高的 GPx 活性（<0.05）。综上所述，结扎改变了公羊精液的蛋白质谱和激素含量，而暴露于恒定光照周期会影响激素浓度和抗氧化酶活性。
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