Sun Ming, Zhao Wenyan, Xie Qingpeng, Zhan Yunhong, Wu Bin
Department of Urology, Shengjing Hospital of China Medical University, Shenyang, 110004, Liaoning Province, PR China.
Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, 110004, Liaoning Province, PR China.
Surg Oncol. 2015 Mar;24(1):28-34. doi: 10.1016/j.suronc.2014.11.002. Epub 2014 Nov 20.
It has been shown that chemotherapy has limited antitumor activity against advanced urothelial bladder cancer (UBC). Consequently, there is an urgent need to develop effective therapeutic methods for patients with advanced UBC. In the present study, the inhibitory effects of lentinan alone, gemcitabine alone, or lentinan combined with gemcitabine on the proliferation of the UBC cell line, T24, were investigated. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, annexin V fluorescein isothiocyanate/propidium iodide staining, and flow cytometry were used to determine the proliferation and apoptosis of T24 cells in each treatment group. Survival-related protein expression was analyzed by western blotting. Increased concentrations of lentinan, or lentinan combined with gemcitabine, positively correlated with decreased T24 cell proliferation. Lentinan combined with gemcitabine chemotherapy significantly inhibited UBC cell proliferation. Gemcitabine has the ability to induce T24 cell apoptosis, and this effect is enhanced when it is combined with lentinan.
研究表明，化疗对晚期尿路上皮膀胱癌（UBC）的抗肿瘤活性有限。因此，迫切需要为晚期UBC患者开发有效的治疗方法。在本研究中，研究了香菇多糖单独、吉西他滨单独或香菇多糖与吉西他滨联合对UBC细胞系T24增殖的抑制作用。采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐法、膜联蛋白V异硫氰酸荧光素/碘化丙啶染色法和流式细胞术来测定各治疗组T24细胞的增殖和凋亡情况。通过蛋白质印迹法分析与生存相关的蛋白表达。香菇多糖或香菇多糖与吉西他滨联合浓度的增加与T24细胞增殖的降低呈正相关。香菇多糖联合吉西他滨化疗显著抑制UBC细胞增殖。吉西他滨具有诱导T24细胞凋亡的能力，当与香菇多糖联合时，这种作用增强。
