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人肺腺癌干细胞的分离、培养与鉴定

Isolation, cultivation and identification of human lung adenocarcinoma stem cells.

作者信息

Zhang DE-Geng, Jiang Ai-Gui, Lu Hui-Yu, Zhang Li-Xin, Gao Xiao-Yan

机构信息

Department of Oncology, Taizhou People's Hospital Affiliated to Medical College of Nantong University, Taizhou, Jiangsu 225300, P.R. China.

Department of Respiratory Medicine, Taizhou People's Hospital Affiliated to Medical College of Nantong University, Taizhou, Jiangsu 225300, P.R. China.

出版信息

Oncol Lett. 2015 Jan;9(1):47-54. doi: 10.3892/ol.2014.2639. Epub 2014 Oct 24.

DOI:10.3892/ol.2014.2639
PMID:25435932
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4246672/
Abstract

Recently, an increasing number of studies have demonstrated that lung cancer is a stem cell disease. However, ideal cell surface markers for isolating stem cells in lung cancer are yet to be identified. In the present study, a cell population with a cluster of differentiation (CD)133 phenotype was successfully isolated from a single cell suspension of lung adenocarcinoma tissue using magnetic-activated cell sorting (MACS) and enriched in a serum-free culture. In comparison to CD133 cells, the CD133 cells exhibited an enhanced capacity for self-renewal and differentiation, and a greater potential for tumor formation, in non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice. Tumors could be induced in NOD/SCID mice by the transplantation of 10 stem-like cells per mouse. The results of the present study demonstrated that CD133 may serve as a specific cell surface marker for lung adenocarcinoma stem cells, and that MACS combined with serum-free culture is an effective method for isolating and enriching lung cancer stem cells.

摘要

最近，越来越多的研究表明肺癌是一种干细胞疾病。然而，用于分离肺癌干细胞的理想细胞表面标志物尚未确定。在本研究中，使用磁珠分选法（MACS）从肺腺癌组织的单细胞悬液中成功分离出具有分化簇（CD）133表型的细胞群，并在无血清培养中进行富集。与CD133阴性细胞相比，CD133阳性细胞在非肥胖糖尿病/重度联合免疫缺陷（NOD/SCID）小鼠中表现出更强的自我更新和分化能力，以及更大的肿瘤形成潜力。通过每只小鼠移植10个干细胞样细胞可在NOD/SCID小鼠中诱导出肿瘤。本研究结果表明，CD133可能作为肺腺癌干细胞的特异性细胞表面标志物，并且MACS结合无血清培养是分离和富集肺癌干细胞的有效方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bdb/4246672/46f701e4abb7/OL-09-01-0047-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bdb/4246672/c98ffca7a335/OL-09-01-0047-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bdb/4246672/b16a0581e80f/OL-09-01-0047-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bdb/4246672/1b28496492f0/OL-09-01-0047-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bdb/4246672/b5c37984c6c2/OL-09-01-0047-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bdb/4246672/89cb5140edcb/OL-09-01-0047-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bdb/4246672/46f701e4abb7/OL-09-01-0047-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bdb/4246672/c98ffca7a335/OL-09-01-0047-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bdb/4246672/b16a0581e80f/OL-09-01-0047-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bdb/4246672/1b28496492f0/OL-09-01-0047-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bdb/4246672/b5c37984c6c2/OL-09-01-0047-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bdb/4246672/89cb5140edcb/OL-09-01-0047-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bdb/4246672/46f701e4abb7/OL-09-01-0047-g05.jpg

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Int J Mol Sci. 2024 Aug 12;25(16):8766. doi: 10.3390/ijms25168766.
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