Chen Wei, Wang Guo-Min
Department of Urology, Zhongshan Hospital of Fudan University, Shanghai 200032, P.R. China.
Oncol Lett. 2012 Apr 1;3(4):791-796. doi: 10.3892/ol.2012.565. Epub 2012 Jan 12.
Cancer stem cells (CSCs) that exhibit tumor-initiating properties have been identified in primary prostate cancer and prostate cancer cell lines. CSCs are the root of tumor metastasis and recurrence even in the cases where they constitute a minority of the tumor mass. In this study, putative CSCs were isolated from the Du145 cell line and an Affymetrix microarray was used to investigate their gene expression profile. The results were validated by real-time polymerase chain reaction (PCR). The results of the microarray indicated that 138 genes were upregulated and 93 genes were downregulated in the CSCs. Certain genes that may be significant in the regulation of CSCs from the Du145 cell line were identified. These results may aid the studying of the mechanisms through which CSCs acquire their distinctive properties.
在原发性前列腺癌和前列腺癌细胞系中已鉴定出具有肿瘤起始特性的癌症干细胞（CSCs）。即使在CSCs占肿瘤组织少数的情况下，它们也是肿瘤转移和复发的根源。在本研究中，从Du145细胞系中分离出假定的CSCs，并使用Affymetrix微阵列研究其基因表达谱。结果通过实时聚合酶链反应（PCR）进行验证。微阵列结果表明，CSCs中有138个基因上调，93个基因下调。鉴定出了某些可能在调控Du145细胞系CSCs中具有重要意义的基因。这些结果可能有助于研究CSCs获得其独特特性的机制。
