Huang Yan, Guo Xiuhua, Wang Hongyang, Zhu Tienian
Department of Oncology, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
Hebei United University Affiliated Hospital, Tangshan, Hebei 063000, P.R. China.
Oncol Lett. 2015 Jan;9(1):252-256. doi: 10.3892/ol.2014.2658. Epub 2014 Nov 3.
Chemotherapy represents an important treatment modality for lung adenocarcinoma. Thymidylate synthase (TS) is an essential enzyme in DNA synthesis, and its overexpression has been associated with reduced sensitivity to antifolate agents. The aim of the current study was to investigate the expression of TS and the effect on prognosis in lung adenocarcinoma patients. Adenocarcinoma and adjacent carcinoma tissues were resected from 100 patients with lung adenocarcinoma and the TS levels were detected by immunohistochemical analysis. The values for overall survival (OS) and disease-free survival (DFS) were determined using the Kaplan-Meier analysis. The results indicated that the TS protein was expressed predominantly in adenocarcinoma tissues, which exhibited higher TS expression compared with the adjacent tissues (P<0.001). The statistical analysis indicated that TS expression was associated with the clinical stage and history of smoking (P<0.05). The Kaplan-Meier analysis results indicated that the DFS and OS in patients with high TS expression levels were significantly shorter compared with those with low expression levels (P<0.05). In conclusion, the results from this study suggested that TS may serve as an independent predictive factor for survival rate, which may indicate the prognosis of lung adenocarcinoma patients.
化疗是肺腺癌的一种重要治疗方式。胸苷酸合成酶（TS）是DNA合成中的一种关键酶，其过表达与对抗叶酸药物的敏感性降低有关。本研究的目的是调查TS在肺腺癌患者中的表达情况及其对预后的影响。从100例肺腺癌患者中切除腺癌组织及癌旁组织，采用免疫组化分析检测TS水平。使用Kaplan-Meier分析确定总生存期（OS）和无病生存期（DFS）的值。结果表明，TS蛋白主要在腺癌组织中表达，与癌旁组织相比，腺癌组织中TS表达更高（P<0.001）。统计分析表明，TS表达与临床分期和吸烟史相关（P<0.05）。Kaplan-Meier分析结果表明，TS表达水平高的患者的DFS和OS明显短于表达水平低的患者（P<0.05）。总之，本研究结果表明，TS可能作为生存率的独立预测因素，这可能提示肺腺癌患者的预后。
