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用铟-111标记单克隆抗体10B、8C和M2A。

Labelling of monoclonal antibodies 10B, 8C, and M2A with indium-111.

作者信息

Reilly R, Sheldon K, Marks A, Houle S

机构信息

Toronto General Hospital, Division of Nuclear Medicine, Ontario, Canada.

出版信息

Int J Rad Appl Instrum A. 1989;40(4):279-83. doi: 10.1016/0883-2889(89)90218-9.

DOI:10.1016/0883-2889(89)90218-9
PMID:2543652
Abstract

Monoclonal antibodies 10B, 8C and M2A were conjugated with cyclic DTPA anhydride to a substitution level of 1-2 mol DTPA/mol antibody. The DTPA-conjugated antibodies were purified by size-exclusion mini-column chromatography and labelled with [111In]In acetate to a specific activity of 37-74 kBq/micrograms (1-2 microCi/micrograms). The radiochemical purity of the antibody preparation was 76-81%. The immunoreactive fraction of the radiolabelled antibodies ranged from 0.17 to 0.31, significantly lower than that of their radioiodinated analogs.

摘要

单克隆抗体10B、8C和M2A与环状二乙烯三胺五乙酸酐结合，取代水平为1-2摩尔二乙烯三胺五乙酸/摩尔抗体。通过尺寸排阻微型柱色谱法纯化二乙烯三胺五乙酸结合的抗体，并用[111In]铟乙酸盐标记，比活度为37-74 kBq/微克（1-2微居里/微克）。抗体制剂的放射化学纯度为76-81%。放射性标记抗体的免疫反应部分为0.17至0.31，明显低于其放射性碘化类似物。