Estevez-Herrera Judith, Pardo Marta R, Dominguez Natalia, Pereda Daniel, Machado Jose D, Borges Ricardo
Biomol Concepts. 2013 Dec;4(6):605-9. doi: 10.1515/bmc-2013-0020.
Chromogranins (Cgs) are acidic proteins implicated in several physiological processes, including the biogenesis and sorting of secretory vesicles, the generation of bioactive peptides, and the accumulation of soluble species inside large dense core vesicles (LDCV). Indeed, Cgs are the main protein component of the vesicular matrix in LDCV, and they are involved in the concentration of soluble species like neurotransmitters and calcium. Experiments using electrochemical techniques such amperometry, patch amperometry, and intracellular electrochemistry have clarified the functional roles of Cgs in the accumulation and release of catecholamines. We have focused this review at a single event of exocytosis of chromaffin cells from three mouse strains lacking Cgs. Accordingly, in this brief review, we will focus on the role of Cgs in maintaining the intravesicular environment of secretory vesicles and in exocytosis, bringing together the most recent findings from studies on adrenal chromaffin cells.
嗜铬粒蛋白（Cgs）是一种酸性蛋白质，参与多种生理过程，包括分泌囊泡的生物发生和分选、生物活性肽的生成以及大致密核心囊泡（LDCV）内可溶性物质的积累。事实上，Cgs是LDCV中囊泡基质的主要蛋白质成分，它们参与神经递质和钙等可溶性物质的浓缩。使用电化学技术（如安培法、膜片安培法和细胞内电化学）进行的实验已经阐明了Cgs在儿茶酚胺积累和释放中的功能作用。我们将本综述聚焦于来自三种缺乏Cgs的小鼠品系的嗜铬细胞胞吐作用的单个事件。因此，在这篇简短的综述中，我们将聚焦于Cgs在维持分泌囊泡的囊泡内环境和胞吐作用中的作用，汇集肾上腺嗜铬细胞研究的最新发现。
